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To find the first one talk to Jin while he is working in the clinic.Then you head up to the light house to pick it up and plant it in the middle of the old tree at goddess spring here is a hint it dies

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Q: Where is the first seedling of hope to save the mother tree on harvest moon tree of tranquility?
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Where is the first seedling of hope on harvest moon tree of tranquility?

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you will find out after you have to find the seedling of hope if you need to know now (spoiler) then keep going down the answer is there if not don't look When you complete Edge's rainbow, talk to Jin and he will tell you about a strange seedling he found by the lighthouse. Go there and pick it up. If you finished Ben's rainbow, you have found his badge, and you can get past the mother bear, go to the harvest goddess spring and plant it. You will then turn around to see that it has died. Then, talk to Calvin and meet him at the mine at Level 10 and he will give you another seedling. Go plant that at the harvest goddess spring. You will once again turn around and see it die then you will have a short talk with Calvin Then, talk to Gill and he will say to speak to his father at the Inn. Talk to him around midnight while he is sitting down. the first response you must pick is the 2nd choice, then the 1st, then the 2nd, and then the 3rd choice and then he will fall asleep. Go to the church the next day and go by the table in the middle of the room. You will find a key. Then, go to Hamilton's house and use the key to unlock the door leading to the backyard. You will find another seedling. Hamilton then comes and tells you how he has been raising the seedling for years. Go to the harvest goddess spring again and plant the seedling. You turn around to find the harvest sprites dancing. They will tell you that they will take care of the seedling until it grows into a new mother tree.


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