Yes
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In "Pokemon Shipwreck" James admitted he liked Jessie, but she seemed to have more important things to do then have a crush on James. In the manga, Jessie and James are married and Jessie is pregnant.
Jessie has had poison and bug types, while James has had poison, grass and psychic types.
: Jessie: Prepare for trouble! (Sometimes changed to match situation/costumes) : James: And make it double! (Sometimes changed to match situation/costumes) : Jessie: To protect the world from devastation! : James: To unite all people within our nation. : Jessie: To denounce the evils of truth and love! : James: To extend our reach to the stars above! : James: Jessie! : Jessie: James! : Jessie: Team Rocket, blast off at the speed of light! : James: Surrender now or prepare to fight! : Meowth: Me-owth! Dat's right! : Wobbuffet: Wooooooooobbuffet! : Chimecho: Chime, Chime! ~Team Rocket Introduction, Kanto to Hoenn ---- : Jessie: Prepare for trouble from the skies! : James: From beyond the stars, a nasty surprise! : Jessie: An evil as old as the galaxy! : James: Sent here to fulfill our destiny! : Meowth: Plus, there's me! : Jessie: To denounce the evils of truth and love! : James: To extend our reach to the stars above! : James: Jessie! : Jessie: James! : Meowth: And Meowth are da names! : Jessie: Anywhere there's peace in the universe, : James: Team Rocket... : Meowth: Will be there... : All:To make everything worse! : Wobbuffet: Wob-buffet! : Chimecho: Chime chime! : Mime Jr: Mime mime mime! ~Team Rocket Introduction, Battle Frontier
No, they arent.
They are not in Pokemon HeartGold