Episode 113
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The Sasuke vs Itachi fight occurred in the Japanese manga and anime, Naruto. Specifically, the battle was fought during the Shpipuden arc of the series.
episode 37 the arlong arc
He kills em before the series starts. But, they show many flashback episodes, sorta as fillers to kinda fill in story plot. In a flashback episode detailing the events of that night, Sasuke mentions he is seven years old. However, the overall timeline is a bit uncertain due to conflicting statements made throughout the series. A brief flashback to the massacre occurs in episode 84. A more detailed one occurs in episode 131, with events leading up to it occurring from the end of episode 128 through 130. These are both flashback's of Sasuke's. Itachi has his own flashback in Shippuden episode 135, and then there is another flashback to that night (Sasuke's again) in Shippuden 142. The whole story behind the massacre is explained in Shippuden episodes 140 and 141.
I believe during an episode during the filler arc with the three tails/crystal user...
Shippuden 20-27 mid Kazekage-retrieval arc. :D