14th Episode of Naruto Shippuden..
While Fighting With Kisame...
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The first time Might Guy fights Kisame is in Naruto in episode 82 where Might Guy fends of Kisame from his comrades. Kisame and Itachi quickly leave after Guy's appearance. The second time Guy fights Kisame is in Naruto Shippuden in episodes 250 and 251
Kisame died in Naruto Shippuden episode 251.
Not yet, but however it is highly possible that he will die against Kisame. Opening the seventh gate is already life-threatening, and it wont be enough to defeat Kisame, even if he is weakened. If it comes down to it, Gai will open the eighth gate, too combat Kisame. The results, of course, will be death. It will turn out like how Asuma died, just to give his team some screen time. Although, this is all just speculation.
kisame appeared in the first naruto anime on episode 81 with his close comrade itachi uchiha who sadly died in the shippuden. but kisame is still alive even in the manga as we speak but I think he is going to die soon in manga see manga 5o6 spoiler he battle might guy
Rock Lee can open Six chakra gates. Rock Lee is not capable of opening all chakra gates. When Rock Lee was fighting Gaara, Kakashi asked Might Guy how many gates he had got up to. Guy said he was up to five gates. Now that he has trained during time-skip, Rock Lee can probably open the sixth chakra gate now.