Read Lucky Star Manga 2. Read pg. 146. Heres a quote from it.
KONATA: "Well, Yeah, that's true, but for now, I have no interest in being a lesbian in real life."
Plus in the anime, when she's talking about romance, it's usually about guys.
But their is also some very suggestive moments in the series. I think its set up to look like kona-chan likes kagami but kagami dosent know it so basically at the end of the series you can draw your own conclusion.
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I'm presuming your referring to Konata Izumi from Lucky Star?
In which case, it isn't, or hasn't, actually been stated in either the anime, video game, or manga, whether Kona-chan is a lesbian.
Although it has been speculated that she's either is or is infact a bisexual (i.e. the Kagami moments).
Hence it's up to your own opinion on her character.