Yes but only for the PS3 and you need to download from the playstion network store.
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you can play as him when you make your third encounter of the scarecrow the illusion will be an alternative of the intro in the gameYou can play as the Joker in the PS3 version.
The Joker was played by the late Cesar Romero(February 15, 1907 - January 1, 1994) .
Harley Quinn (play on the word harlequin) first appeared in "Batman: The Animated Series" epidsoe "Jokers Favor". Since then she has appeared in many other series including comic books. Her first comic book was "The Batman Adventures: Mad Love", but it was written and drawn by Paul Dini and Bruce Timm (who were 2 producers in of the animated series and Harley's creators) the comic book revealed Harley's origins as an Arkham Asylum psychiatrist who fell in love with the Joker. The story was widely praised and won the Eisner and Harvey Awards for Best Single Issue Comic of the Year. Since then she has appeared in animated series such as "The Batman" and a small cameo appearance at the end of "Batman Beyond: Return of the Joker"
Batman Forever
Lewis Wilson play Batman in the first Batma n serial movie in 1933. Then Robert Lowery in 1939 in Batman and Robin : Relentless Crusaders for Justice!it was the year 1943 in which Lewis Wilson frist played the role of Batman in the first 15 serials. and 1949 in which Robert lowery played Batman in the second set of 15 serials.Unless you're talking purely about the comics continuity, in which case there's a sort of trick answer. Bruce Wayne's father, Thomas Wayne, dressed up like a "Bat-Man" for a costume party when Bruce was a little boy (he still has the costume), but Bruce himself was the first person to use a Batman persona to fight crime. When Bane broke Batman's back, Azreal breifly was Batman.