We don't know the answer. There was a famous Greek poet, Sappho, who was born on the island of Lesbos, somewhere around 630 BCE. Sappho wrote many poems about the beauty of other women.
Here is a piece addressed to a bride getting married:
And how you laugh your charming laugh. Why it
makes my heart flutter within my breast,
because the moment I look at you, right then, for me,
to make any sound at all won't work any more.
My tongue has a breakdown and a delicate
- all of a sudden - fire rushes under my skin.
With my eyes I see not a thing, and there is a roar
that my ears make.
Sweat pours down me and a trembling
seizes all of me; paler than grass
am I, and a little short of death
do I appear to me.
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It is impossible to determine who the first lesbian was, as same-sex relationships have existed throughout history across various cultures. The concept of sexual orientation as we know it today is a modern construct.