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ProfessorLysis is the physical breakdown of a cell membrane, releasing its contents, while lysate is the resulting cell contents released after lysis. Lysis refers to the process of breaking open cells, whereas a lysate is the mixture of cellular components released from the broken cells.
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Use of chloroform in plasmid isolation?
Chloroform is used in plasmid isolation to partition cellular components. It is often added to a mixture of bacterial lysate and alkaline lysis reagent to help separate the plasmid DNA from proteins, genomic DNA, and other cellular debris. After centrifugation, the chloroform helps to separate the aqueous and organic phases, allowing for collection of the purified plasmid DNA from the aqueous phase.
What does lysis solution do?
Lysis solution usually contains multiple components which will disrupt cellular membranes, inactivate proteins, and stabilize a nucleic acid component.
How much difference between 2oz and 1.69 oz?
The difference between 2 oz and 1.69 oz is 0.31 oz.
What is the difference in weight between platinum and gold?
The difference in weight between platinum and gold is that platinum is denser and heavier than gold.
What is the difference between 102 and 103?
well, 102 is 1 less then 103, there different numbers
