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Both are forms of luminescence that result from the absorption of energy from a particular source and then re-emit light during the de-excitation of the electrons within the material in question occurs. The difference lies in what the source of this energy is.
Scintillation is the result of the absorption of energy from ionized radiation.
Fluorescence is the result of the absorption of energy from strictly, electromagnetic radiation.
For instance, if you shoot an electron or an alpha particle through a material and the result is a glowing substance, then scintillation is occurring. If you just shine light ata material and it re-emits light, then it's fluorescence.
Since high energy photons, gamma radiation, can generate scintillation and photons are electromagnetic in nature, then it is possible to say that in this instance, scintillation is fluorescence.
Both re-emit light that is generally of less energy than the energy the material is absorbed.
E = hv where E is Energy, h is Plank's constant, and v is frequency.
Given c=wv where w is the wavelength then Energy is inversely proportional to the wavelength of light.
E = hc/w
So the less energy, the larger the wavelength or longer the wavelength is emitted.
This would suggest a red shift in the re-emission process.
Fluorescence occurs when a material absorbs light energy and re-emits it at a longer wavelength, while scintillation produces flashes of light when ionizing radiation interacts with a crystal material. Fluorescence typically involves lower energy transitions within atoms or molecules, whereas scintillation involves higher energy interactions that produce visible light.
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What type of radiation is best detected by a scintillation counter?
Gamma radiation is best detected by a scintillation counter due to its ability to interact with scintillation materials and produce light pulses that can be detected.
What is Relative Fluorescence Intensity?
Relative fluorescence intensity is a measure of the amount of fluorescence emitted by a sample compared to a reference sample. It is often used in fluorescence spectroscopy to quantify the fluorescence signal from a sample relative to a standard for comparison and analysis.
How do you calculate the relative intensity of fluorescence?
The relative intensity of fluorescence can be calculated by dividing the fluorescence intensity of the sample of interest by the fluorescence intensity of a reference standard under the same conditions. This ratio provides a measure of the relative fluorescence properties of the sample compared to the reference standard.
Phosphorescence is similar to what other source of light?
Phosphorescence is similar to fluorescence in that both involve emission of light by materials after they have absorbed energy. The main difference is the time scale: fluorescence is immediate, while phosphorescence has a delay before light is emitted.
What causes fluorescence?
Fluorescence occurs when a molecule absorbs light energy and then quickly releases it as lower-energy, longer-wavelength light. This phenomenon is typically caused by specific chemical structures within a molecule that allow it to absorb light and emit fluorescence.
What is the difference between spectrophotometer and fluorimeter?
A spectrometer shoots light through a sample and detects absorbance while a fluorimeter detects the intensity of fluorescence of a given sample.
What is the main detector of a scintillation counter?
The main detector of a scintillation counter is a photomultiplier tube. This tube converts the light produced by scintillation events into an electrical signal that can be measured and analyzed.
What compound between phenolphthalein and fluorescein are expected to have the greater quantum yield of fluorescence?
phenolphthalein will have a greater quantum yield because dissolved oxygen can reduce the fluorescence intensity
What is difference between an HPLC UV detector and a fluorescence detector?
HPLC UV detectors measure absorbance of UV light at a specific wavelength, while fluorescence detectors measure the emission of light at a longer wavelength after excitation with UV light. Fluorescence detectors are more sensitive and selective than UV detectors, but may require additional steps such as derivatization for certain compounds.
What type of radiation is best detected by a scintillation counter?
Gamma radiation is best detected by a scintillation counter due to its ability to interact with scintillation materials and produce light pulses that can be detected.
Is fluorescence a reliable mineral?
Fluorescence is a property not a mineral.
What is Relative Fluorescence Intensity?
Relative fluorescence intensity is a measure of the amount of fluorescence emitted by a sample compared to a reference sample. It is often used in fluorescence spectroscopy to quantify the fluorescence signal from a sample relative to a standard for comparison and analysis.
What is the difference between raman effect and flueroscence?
The Raman effect is the inelastic scattering of light by molecules, resulting in a shift in wavelength. Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. Both phenomena involve interactions between light and molecules but differ in the mechanism of light emission.
What can be used to compare the amount of radioactivity labeled DNA in two or more samples?
Scintillation counter
How do you calculate the relative intensity of fluorescence?
The relative intensity of fluorescence can be calculated by dividing the fluorescence intensity of the sample of interest by the fluorescence intensity of a reference standard under the same conditions. This ratio provides a measure of the relative fluorescence properties of the sample compared to the reference standard.
What is the principle of fluorescence?
The principle of fluorescence spectroscopy is the interaction with light image.
Phosphorescence is similar to what other source of light?
Phosphorescence is similar to fluorescence in that both involve emission of light by materials after they have absorbed energy. The main difference is the time scale: fluorescence is immediate, while phosphorescence has a delay before light is emitted.
