flourescence is more sensetive than UV detection
Yes, you can use a C18 column and methanol as a mobile phase with fluorescence detector. Fluorescence detector is generally used as it can detect the presence of compounds at a very low concentration.
HPLC UV detector is a component used in high-performance liquid chromatography (HPLC) to monitor eluent absorbance, while a spectrophotometer UV detector is a standalone instrument used to measure the absorption of light at different wavelengths. HPLC UV detectors are specifically tailored for chromatography applications, whereas spectrophotometer UV detectors are more versatile and used for various analytical purposes.
An RID (Refractive Index Detector) for HPLC works by measuring changes in refractive index caused by the presence of analytes eluting from the column. As analytes pass through the detector cell, they displace the mobile phase, causing changes in refractive index that are detected and converted into a signal. The signal is then plotted against retention time to create a chromatogram that can be used to identify and quantify analytes in the sample.
In HPLC, a standard is a known compound with a defined chemical structure and purity used for comparison and identification purposes. Standards are essential for calibrating instruments, determining retention times, and quantifying unknown compounds in samples during analysis.
Yes, HPLC can be used to analyze histamine and TVB-N (Total Volatile Basic Nitrogen) in food samples. HPLC is a sensitive and selective technique that can separate and quantify various compounds, including histamine and TVB-N, based on their chemical properties. Pre-column derivatization may be required for some compounds to enhance their detection sensitivity in HPLC analysis.
Yes, you can use a C18 column and methanol as a mobile phase with fluorescence detector. Fluorescence detector is generally used as it can detect the presence of compounds at a very low concentration.
HPLC UV detector is a component used in high-performance liquid chromatography (HPLC) to monitor eluent absorbance, while a spectrophotometer UV detector is a standalone instrument used to measure the absorption of light at different wavelengths. HPLC UV detectors are specifically tailored for chromatography applications, whereas spectrophotometer UV detectors are more versatile and used for various analytical purposes.
We can quantitatively analyse pregabalin on hplc with uv detector, wavelength will be 210 n.m. and mobile phase will be 5 % acetonitrile. standard & sample solution preparation should be in mobile phase.
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
PDA - Photo diode array UV- Ultra violet with use of PDA detector, we can measure the area or height of particular peak at different wavelengths ranging from 200 to 800 nm by injecting the solution at once. Where as in uv detector we can measure the area or height of the peak only at two different wavelengths. But that wavelengths also to be selected before injecting the solution.
Of course.... there isn´t problem....
1. Flow rate 2. Temp. of column 3. Detector function 4. Resolution
An RID (Refractive Index Detector) for HPLC works by measuring changes in refractive index caused by the presence of analytes eluting from the column. As analytes pass through the detector cell, they displace the mobile phase, causing changes in refractive index that are detected and converted into a signal. The signal is then plotted against retention time to create a chromatogram that can be used to identify and quantify analytes in the sample.
In HPLC, a standard is a known compound with a defined chemical structure and purity used for comparison and identification purposes. Standards are essential for calibrating instruments, determining retention times, and quantifying unknown compounds in samples during analysis.
Post run in HPLC refers to the time after the completion of a chromatographic analysis where the system continues running to ensure that any remaining compounds are fully flushed out of the column and detector to prevent contamination and achieve a clean baseline for subsequent runs. It is an important step to maintain the integrity and performance of the HPLC system.
Yes, HPLC can be used to analyze histamine and TVB-N (Total Volatile Basic Nitrogen) in food samples. HPLC is a sensitive and selective technique that can separate and quantify various compounds, including histamine and TVB-N, based on their chemical properties. Pre-column derivatization may be required for some compounds to enhance their detection sensitivity in HPLC analysis.
Reverse phase and normal phase HPLC techniques differ primarily in the polarity of the stationary phase and mobile phase. In reverse phase HPLC, the stationary phase is nonpolar and the mobile phase is polar, while in normal phase HPLC, the stationary phase is polar and the mobile phase is nonpolar. This polarity difference affects the retention and separation of compounds in the sample.