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What is difference between an HPLC UV detector and a fluorescence detector?
Wiki User
∙ 12y ago
flourescence is more sensetive than UV detection
Wiki User
∙ 12y ago
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What is Difference Between HPLC UV detector Spectrophotometer uv detector?
hi, could any one answer what is the difference between hplc uv detector and spectrophotometer uv detector ? my id is debendra_mallick2002@yahoomail.com
Can you use a C18 column for HPLC with fluorescence detector and methanol as the mobile phase?
Yes, you can use a C18 column and methanol as a mobile phase with fluorescence detector. Fluorescence detector is generally used as it can detect the presence of compounds at a very low concentration.
How does an RID detector work for HPLC?
boh
Definition of standard in HPLC?
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
Does HPLC can read histamine and TVB-N?
post column derivatisation with ortho-phthal-dialdehyde and fluorescence detection
What is Difference Between HPLC UV detector Spectrophotometer uv detector?
hi, could any one answer what is the difference between hplc uv detector and spectrophotometer uv detector ? my id is debendra_mallick2002@yahoomail.com
Can you use a C18 column for HPLC with fluorescence detector and methanol as the mobile phase?
Yes, you can use a C18 column and methanol as a mobile phase with fluorescence detector. Fluorescence detector is generally used as it can detect the presence of compounds at a very low concentration.
How does an RID detector work for HPLC?
boh
Definition of standard in HPLC?
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
Does HPLC can read histamine and TVB-N?
post column derivatisation with ortho-phthal-dialdehyde and fluorescence detection
How you can analyze pregabalin on HPLC with UV detector?
We can quantitatively analyse pregabalin on hplc with uv detector, wavelength will be 210 n.m. and mobile phase will be 5 % acetonitrile. standard & sample solution preparation should be in mobile phase.
What is difference between specificity and selectivity in hplc method validation?
specificity and selectivity
Why is Erbium perchlorate is used in calibration of HPLC?
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
What difference between pda and uv detector in hplc?
PDA - Photo diode array UV- Ultra violet with use of PDA detector, we can measure the area or height of particular peak at different wavelengths ranging from 200 to 800 nm by injecting the solution at once. Where as in uv detector we can measure the area or height of the peak only at two different wavelengths. But that wavelengths also to be selected before injecting the solution.
Can C18 column for HPLC be used with UV and visible spectra detector and Acetonitril as mobile phase?
Of course.... there isn´t problem....
What are the HPLC Calibration parameters and elaborate it?
1. Flow rate 2. Temp. of column 3. Detector function 4. Resolution
What is difference bet GLC and HPLC?
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
