In qualitative PCR specific DNA fragment is detected while in quantitative PCR our target DNA sequence not only is detected but its amount is determined (after reaction we can calculate the amount of DNA we had in our sample)
Difference between real time PCR and reverse transcription PCR is as follows:- 1. Real time PCR is donated as qPCR and on the other hand reverse transcription PCR is denoted as RT-PCR. 2. In qPCR, the template used is single strand DNA strand whereas in the RT-PCR, the template used in process is single strand of RNA. 3. The real time PCR enables both quantification as well as detection of the DNA in the real time whereas the RT-PCR enables only the quantification of the RNA and it is little bit slower process then the qPCR as it first produce the cDNA from the template RNA strand and then process it in the similar fashion as the traditional PCR.
PCR uses Taq polymerase (a type of DNA polymerase that comes from the thermophilic bacterium, Thermus aquaticus).The difference between that version of the enzyme and the one that our cells use is that Taq polymerase functions at much higher temperatures, allowing it to withstand PCR temperatures for thermal cycling.
to check is there any contamination in pcr products
It Inhibits the PCR reaction by chelating the magnesium ions.
The choice of primers controls which DNA is amplified in PCR.
what is the difference between PCR simplex and multiplex
Certainly rt-PCR is qualitative and can also theoretically be quantitative. Anneal the RNA to get a 1:1 RNA to DNA copy, then proceed with quantitative PCR.
PCR: Passenger Car Radial TBR: Truck and Bus Radial A TBR tire can handle a heavier load than a PCR tire, and it's usually bigger.
Difference between real time PCR and reverse transcription PCR is as follows:- 1. Real time PCR is donated as qPCR and on the other hand reverse transcription PCR is denoted as RT-PCR. 2. In qPCR, the template used is single strand DNA strand whereas in the RT-PCR, the template used in process is single strand of RNA. 3. The real time PCR enables both quantification as well as detection of the DNA in the real time whereas the RT-PCR enables only the quantification of the RNA and it is little bit slower process then the qPCR as it first produce the cDNA from the template RNA strand and then process it in the similar fashion as the traditional PCR.
: Differentiate between quantitative and real time PCR.
PCR allows amplification of DNA for a specific gene, after too many cycles of PCR the result will reach saturation, basically meaning all of the DNA has been amplified. Conventional PCR will basically tell you whether or not a gene is expressed in your sample. This can be done semi-quantitavely if the PCR is performed for a low number of cycles, ie it will tell you whether one sample expresses more of your gene of interest than another sample. The results are seen by separating the PCR products by agarose gel/ethidium bromide electrophoresis. Real-time PCR will record exactly what cycle of PCR a detectable level of amplified product became detectable, giving a far more accurately quantifiable estimation of gene expression.
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
PCR uses Taq polymerase (a type of DNA polymerase that comes from the thermophilic bacterium, Thermus aquaticus).The difference between that version of the enzyme and the one that our cells use is that Taq polymerase functions at much higher temperatures, allowing it to withstand PCR temperatures for thermal cycling.
Allele specific PCR is used in the process of DNA fingerprinting where specific alleles Can be amplifies and the degree of similarity or difference between individuals can be determined.
PCR is the abbreviation for polymerase chain reaction. It is similar to recombinant DNA technology in that both have the ability to sequence DNA.
The use of dNTP is PCR and multiplex PCR