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Difference between real time PCR and reverse transcription PCR is as follows:-

1. Real time PCR is donated as qPCR and on the other hand reverse transcription PCR is denoted as RT-PCR.

2. In qPCR, the template used is single strand DNA strand whereas in the RT-PCR, the template used in process is single strand of RNA.

3. The real time PCR enables both quantification as well as detection of the DNA in the real time whereas the RT-PCR enables only the quantification of the RNA and it is little bit slower process then the qPCR as it first produce the cDNA from the template RNA strand and then process it in the similar fashion as the traditional PCR.

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Q: What is the defference between Real-time PCR and reverse transcriptase PCR?
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