There are no specific "signs" to indicate that a person is a lesbian. Some lesbians prefer to wear items of jewellery or clothing indicative of their sexual preference, such as rainbow colours, symbols of sexuality, certain hairstyles etc. But as many of these traits are shared by other, non lesbian females, it is wise not to make assumptions. A female that is in a relationship with another female is in all likelihood a lesbian, or bisexual.
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Sexual orientation is not always apparent based on outward appearance or behavior. The only way to know someone's sexual orientation is if they choose to share that information with you. It is important to respect people's privacy and not make assumptions about their identity based on stereotypes or superficial cues.