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Not to any significant degree.

As implied by the old adage "It takes 42 muscles to frown and only 4 to smile but it doesn't take any to just sit there with a dumb look on your face", smiling does require that you tense certain muscles, which would use a tiny amount of energy. Perhaps enough to allow you to eat a sesame seed without gaining weight.

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Q: Do you lose calories smileing
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