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I've been taking levothyroxine for hypothyroidism my whole life and though I've never been pregnant, I've never read anything or been told by my doctor that the medication would affect a pregnancy test.
i see no reason why it would affect a pregnancy test because levothyroxine is synthetic replacement of thyroid hormones that would otherwise already be in your body if your thyroid gland was working properly. pregnancy tests are testing for a specific pregnancy hormone and shouldn't be affected by levothyroxine.
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