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I had a bacterial unknown of M. luteus in my microbiology lab. M. luteus is a Gram positive cocci (as seen by a gram stain). A good definitive test for Gram + cocci is the catalase test. M. luteus is catalase positive. Then a nitrate test can be performed to determine that M. luteus is nitrate negative.
Those alone should be enough to confirm M. luteus.
Biochemical tests to confirm Micrococcus luteus include catalase positive, coagulase negative, positive reactions for oxidase, nitrate reduction, and maltose fermentation tests. Additionally, M. luteus is non-motile, exhibits yellow pigmentation, and grows well on nutrient agar.
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