Master Chief is most definitely male. In the games, his voice is clearly male, and he is always referred to as male. In addition, the official backstory has him being a boy named "John" prior to him being selected to become a Spartan.
Master Chief is a rank not a name, in the halo trilogy the main character is a spartan by the name of John-117 who is a male, but there are female master chiefs.
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master chief is totaly NOT A GIRL.OfcnStupid person
No ok first thing of all master chief is a girl!
Master Chief is a male.
it is a rank in the spartan army and John (master chief) is not only the last spartan but he is also the only master chief
Master Chief is a Master Chief in the UNSC (A fictional branch of the military) and is the highest enlisted rank attainable.