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You can only get Knuckles in Sonic 2 if you stack Sonic 2 and Sonic and Knuckles on the Genesis. Otherwise Knuckles cannot be played in the game.
1:Sonic the Hedgehog 2 2:Sonic Adventure DX 3:Sonic Advance 2 4:Sonic 3 & Knuckles 5:Sonic the Hedgehog 5: Sonic the Hedgehog 4: Sonic Advance 3 3: Sonic Adventure 2: Sonic & knuckles 1: Sonic CD 5: Sonic the Hedgehog 3 4: Sonic the Hedgehog 3: Sonic the Hedgehog (2006) 2: Sonic and the Secret Rings 1: (what? not sonic cd?) Sonic Adventure
There isn't one that is just Sonic the hedgehog 2, but there is the Sonic Classic Collection which has 1,2,3, and Sonic & Knuckles
In Knuckles in Sonic the hedgehog 2, collect all the 7 Chaos Emeralds and jump with over 50 rings to transform into Super Knuckles.
Espio is playable in Sonic heroes,Knuckles Chaotix,Sonic the fighters,Shadow the hedgehog and Sonic Rivals 2.