Jolene is not a lesbian, as she has been dating several guys in recent years. She has however talked about especially one incident where she had been intimately involved with am unnamed girlfriend back in 2002.
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Juliana Jolene's birth name is Crystal Beutz.
Everyone in this world can be, even the most straight ones.
Dolly Parton has a song Jolene, covered by many artist. The version of the Whites Stripes is well known. Ray La Montagne has also a song called Jolene.