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Cloning VectorsVectorMaximum Insert sizeApprox. No. of clones required in libraryAdvantages?Disadvantages?lambda20 kb5 x 105easy to construct libraries,
relatively stable insertsmany clones required
hard to prepare DNA from clonescosmid45 kb2 x 105easy to construct libraries
easy to prepare DNA from clonesnot always stableYAC1 Mb104few clones requiredvery prone to rearrangement,
difficult to constructBAC> 500 kb5 x 104few clones required
very stablesingle copy origin of replication therefore harder to prepare DNA
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Who is responsible for human genome project?
On May 9, 1998, Crag Venter, president of The Institute for Genomic Research (TIGR), announced his company was teaming up with pharmaceutical giant Perkin-Elmer to sequence the human genome
What is an application of the human genome project that involves the insertion of normal genes int o cells with defective genes in an attemp to correcy genetic disorders?
Gene Therapy
Difference between a project that has a fixed delivery day and one that has a fixed limit on resource usage?
A project that a fixed delivery day is one that must be delivered by a certain date, and for that the manager shall make decisions that will lead to that accomplishment. One example is if a certain medical product is to be launched in a new market. A project with a fixed delivery day could be in place for regulation and compliance, and because of that the project manager might have to "cut corners" or go over budget in order to accomplish that objective. On the other hand a project with a fixed limit on resource usage is one that must be completed within the limits imposed related to resources - either financial or human. Let's say a company is looking to improve their customer service, but dispose of limited financial and human resources for training of personnel. With that in mind, the project manager has to complete the project as resources become available, vying for the earliest possible date within said reality. It's important for the project manager to know the difference so that he/she can make decisions and lay out a work map that go in accordance with the limitations of the project.
What are examples of appropriate requirements for the project?
The site must allow users to search for course curriculum The site must meet the web accessibility recommendations of the Equality and Human Rights Comminssion The home page of the site must have a response time not exceeding five seconds
Are you a human or a robot?
i am a human
