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The voting public in each territory that came up for statehood.
Because of the enormous new territories acquired from Mexico. These extended so far either side of the Missouri line that the Missouri Compromise was not workable. This intensified the debate over new territories joining the Union as slave-states.
Calfornia extended so far either side of the Missouri line (North of which slavery was illegal) that it rendered the Missouri Compromise unworkable, so a new one had to be worked out. It was a patched-up deal, and it did not last.
I believe you are a bit mixed up. The South wanted slavery.
The differences between the north and south they have different economise. The North relied on industry and technology because of the bad soil so they couldn't farm. The south relied on farming and agriculture because of their Rich soil! Also, because of this big economical difference slavery was an issue. in fact slavery was the biggest issue and was the major cause of the civil war! The south wanted slavery because they were always farming. However, the North had no need for slavery because of their industries. But even though the north didn't need slavery they were against it. They morally believed it was wrong and wanted to end it. The south was not going to give up slavery so tensions rose there for causing big tensions leading to the civil war!