0
Add your answer:
Earn +20 pts
Based on the results of what conclusions can be drawn about the action of the enzyme as the temperature increase?
enzyme A becomes less effective earlier than enzyme B enzyme b stays effective at higher temperatures than enzyme a
What does the helicase enzyme do?
Helicase is an enzyme involved in DNA replication. It unwinds and unzips the parental DNA strand.
Short-chain triglycerides found in foods such as butterfat molecules in milk are split by a specific enzyme in preparation for absorption what enzymes is responsible?
lipase enzyme
Some medicines work by blocking enzyme activity in pathogens These medicines are effective because they?
have a shape that fits into the enzyme
If an enzyme in solution is saturated with substate What is the most effective way to obtain yield of produtct?
If an enzyme solution is saturated with substrate the most effective way to obtain a faster yield of products is to increase the enzyme concentration. An alternative is to increase the temperature.
What conclusion can be drawn about the action of the enzymes as the temperature increase?
enzyme A becomes less effective earlier than enzyme B enzyme b stays effective at higher temperatures than enzyme a
How much quantity of glucose oxidase for 1 IU?
The quantity of glucose oxidase for 1 IU can vary depending on the specific enzyme preparation and assay method used. Generally, 1 IU of glucose oxidase is defined as the amount of enzyme that catalyzes the oxidation of 1 micromole of glucose per minute under specific conditions. It is typically around 1 microgram of enzyme, but it is important to refer to the manufacturer's instructions for the specific enzyme preparation being used.
How can you tell if isolated RNA is free of DNA?
There is a DNA killing step in RNA isolation by the enzyme DNase I. This will make sure your preparation is free of DNA.
Is acid is bad for an enzyme?
Yes, acids can denature proteins and enzymes, making them less effective or disabling or destroying them completely
What does the wrong pH do to the shape of an enzyme?
A wrong pH can affect the shape of an enzyme by disrupting the interactions between the enzyme's amino acid residues, leading to a change in the enzyme's conformation. This can affect the enzyme's active site, making it less effective at catalyzing reactions.
What would be unlikely to contribute to the substrate specificity of an enzyme?
The size of the enzyme's active site would not contribute significantly to substrate specificity. Substrate specificity is typically determined by the shape, charge, and chemical properties of the active site that can properly bind to the substrate.
Why phosphate buffer is used in determination of enzyme activity?
Phosphate buffer is commonly used in enzyme assays because it maintains a stable pH (around 7.0) which is optimal for most enzyme activities. This helps to ensure that the enzyme functions at its maximum capacity and that the assay results are reliable. Additionally, phosphate buffer is compatible with many enzymatic reactions and is cost-effective.
