0
Retention time is the time it takes for a compound to travel from the injection point to the detector in chromatography. Relative retention time is the ratio of the retention time of a compound to that of a reference compound in the same chromatographic system. It is used for comparing the behavior of different compounds on the same chromatographic column.
Add your answer:
Earn +20 pts
Why retention time vary during isocratic analysis?
Retention time can vary during isocratic analysis due to changes in experimental conditions such as flow rate, temperature, or column stability. Retention time is influenced by the interactions between the analyte, stationary phase, and mobile phase, which can fluctuate during the analysis leading to variations in retention time.
How do you define the retention value of a chemical species?
The retention value of a chemical species refers to its ability to remain in a specific location or system, often used in the context of chromatography where it indicates how strongly a compound is retained on a stationary phase compared to the mobile phase. This is quantified by the retention factor (k) or retention time, which is calculated based on the time taken for the compound to travel through the column compared to the mobile phase.
What will be the effect of pH on retention time?
Changes in pH can alter the degree of ionization of analytes, affecting their interaction with the stationary phase and mobile phase. This can impact retention time by changing the polarity and charge of the analytes, leading to variations in their retention on the column. Different analytes may respond differently to changes in pH, resulting in shifts in retention times.
What is allowable range of retention time of a peak in hplc analysis if retention time is about 5 minutes?
The allowable range of retention time in HPLC analysis is typically ±0.2-0.3 minutes. Therefore, for a peak with a retention time of 5 minutes, the acceptable range would be around 4.7-5.3 minutes. Any significant deviation outside this range may indicate issues with the analysis.
How buffer concentration effects on retention time in hplc method?
Buffer concentration can affect retention time in HPLC by influencing the pH of the mobile phase, which can in turn impact interactions between the analyte and stationary phase. Higher buffer concentrations can alter the ionization state of the analyte, leading to changes in its retention time. Additionally, buffer concentrations can also affect peak shape and resolution in the chromatogram.
Can relative retention time changed?
Yes, relative retention time can change based on various factors such as changes in column temperature, flow rate, and mobile phase composition. These changes can affect the interaction between the analyte and stationary phase, thereby altering the relative retention time.
Why relative retention time used for related substances?
Relative retention time is used for related substances in chromatography because it allows for easy comparison of retention times between different compounds on the same chromatographic system. By expressing retention times relative to a reference compound, such as the main peak of interest, it helps in identifying and quantifying related substances accurately and efficiently.
What is difference between retention time and detention time?
Retention time is the time of passing material through the volume of the drum with determined flowrate :Retention time=V/Qwhich Q : flowrateand V : Volumebut Detention time is the time that materials stay in drum
How to calculate Relative retention time?
Divide the retention time of the peak of ineterest (ex. 14.8 min.) by the retention time of the main peak (ex. 15.9 min.) 14.8/15.9 = 0.93 Therefore your RRT is 0.93 Remember, any peak with an RRT <1 elutes before the main peak, and any peak with an RRT >1 elutes after the main peak! What is RRT & RRF in hplc
What is RRT and RRF in hplc?
In HPLC RRT means Relative Retention Time and RRF is Relative Response Factor
How will calculate rrf in hplc?
To calculate relative retention factor (RRF) in HPLC, you need to divide the retention time of the compound of interest by the retention time of the reference compound. The formula is RRF = (Retention time of compound of interest) / (Retention time of reference compound). This value helps in comparison and identification of compounds in the chromatogram.
What is the difference between relative and absolute time?
Okay, darlin absolute time is the time exactly, like the absolute time is 9:33. Relative time is rounded basically, you probly hear people say its relatively 'round 9:30. Hope that helps.
What property of the product molecules seems to be the most important in determinining relative retention time?
The most important property in determining relative retention time is the molecular size and shape of the product molecules. Larger or more complex molecules tend to elute later in gas chromatography compared to smaller or simpler molecules with similar chemical properties.
What is the time difference between Toronto and negril Jamaica?
There is no time difference between them.
What is the period of difference between the lunar and solar year called?
The period of difference between the lunar and solar year is called the epact. This is the time it takes for the moon to return to the same phase relative to the sun, which is approximately 19 solar years.
What is the time difference between cairns and Brisbane?
there is no difference between the time of the two cities
What is the time difference between Singapore and yunnan?
There is no time difference between Singapore and China.
