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There are two main classifications of culture medianamely defined and undefined. An undefined culture mediumcontains a basic source of aminoacids and nitrogen which are the essential requirements for growth of microorganisms. Common examples of the constituents are beef and yeast extracts. The exact composition and percentage of the individual amino acid or nitrogen providing source is unknown and this is used for basic culture and maintenance of strains. An undefined medium is also called a basal or complex medium. In addition to the aminoacid and nitrogen source it also contains a source for glucose, water and other essential salts.

A defined culture medium or synthetic medium is one that contains salts and a glucose source and other components based on the requirements of culture. It contains no yeast or animal extracts. Each of the components used are accurately weighed and the percentage known.

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Q: What is the difference between defined and undefined culture media?
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