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Turbidity measures the degree of light is scattered by suspended particulate material and soluble colored compounds in the water.
Absorbance measures the amount of light absorbed by the constituents in the water with a specified wavelength.
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What is the difference between optical density and absorbance in spectrophotometry?
In spectrophotometry, optical density and absorbance both measure how much light is absorbed by a sample. However, optical density is a logarithmic measure of the ratio of incident light to transmitted light, while absorbance is a linear measure of the amount of light absorbed by the sample.
What is the difference between absorbance and transmittance?
Absorbance measures the amount of light absorbed by a sample, while transmittance measures the amount of light that passes through a sample. Absorbance is calculated as -log(T), where T is transmittance. Absorbance is commonly used in spectrophotometry to quantify the concentration of a substance in a solution.
What is the relationship between the optical density (OD) and absorbance in spectrophotometry?
In spectrophotometry, optical density (OD) and absorbance are directly related. As the OD increases, the absorbance also increases. This means that a higher OD value indicates a higher absorbance of light by the sample being measured.
Why does a turbidity current move beneath the clear water that surrounds it?
A turbidity current is denser due to the sediment it carries, causing it to sink beneath the clearer, lighter water. This density difference results in the turbidity current flowing along the seafloor, transporting sediment and creating undersea canyons and deposits.
What is the relationship between absorbance intensity of incident radiation and intensity of transmitted radiation?
The relationship between absorbance intensity of incident radiation and intensity of transmitted radiation is inverse. As absorbance increases, transmitted intensity decreases. This is due to the absorption of light energy by the material, leading to a reduction in the amount of light passing through it.
Can a turbid sample be measured for absorbance in UV spectrophotometer?
Yes, turbidity can interfere with absorbance readings in a UV spectrophotometer by causing scattering of light. To accurately measure absorbance in a turbid sample, the turbidity would need to be reduced or removed before analysis, for example by centrifugation or filtration.
What type of instrument is available for measurement of bacterial growth on the basis of turbidity?
A spectrophotometer can be used to measure bacterial growth based on turbidity. It detects changes in light absorbance caused by the presence of bacteria in a liquid culture, with higher turbidity indicating more bacterial growth.
What is difference between specific absorbance and absorbance interms of spectroscopy?
specific absorbance- it is absorbance in a solution containing one gm of substance in 100 ml solvent in 1cm shell. so it is having a difference with absorbance which is negative logarithm of incident light to the transmitted light. divya.chakraborty@gmail.com
What is the difference between spectrophotometer and fluorimeter?
A spectrometer shoots light through a sample and detects absorbance while a fluorimeter detects the intensity of fluorescence of a given sample.
What is difference between suspended solid and turbidity?
Suspended solids are particles present in water that can settle out over time, while turbidity is a measure of the cloudiness or haziness of water due to particles in suspension. Suspended solids are the actual particles in the water, while turbidity is a measure of how those particles affect the clarity of the water.
What is the relationship between wavelength and absorbance in a spectrophotometry experiment?
In a spectrophotometry experiment, there is an inverse relationship between wavelength and absorbance. This means that as the wavelength of light increases, the absorbance decreases, and vice versa.
What is the difference between optical density and absorbance in spectrophotometry?
In spectrophotometry, optical density and absorbance both measure how much light is absorbed by a sample. However, optical density is a logarithmic measure of the ratio of incident light to transmitted light, while absorbance is a linear measure of the amount of light absorbed by the sample.
What is the different between primary absorbance and secondary absorbance in fluorescence?
in primary light absorbed by outer molecule while in secondary re-absorbance occurs
What is the difference between absorbance and transmittance?
Absorbance measures the amount of light absorbed by a sample, while transmittance measures the amount of light that passes through a sample. Absorbance is calculated as -log(T), where T is transmittance. Absorbance is commonly used in spectrophotometry to quantify the concentration of a substance in a solution.
Can you explain the relationship between absorbance and wavelength in a graph?
In a graph, absorbance is typically shown on the y-axis and wavelength on the x-axis. The relationship between absorbance and wavelength is that as the wavelength of light increases, the absorbance generally decreases. This is because different substances absorb light at specific wavelengths, so the absorbance of a substance can vary depending on the wavelength of light being used.
What is the relationship between the absorbance of tryptophan and its concentration in a given solution?
The relationship between the absorbance of tryptophan and its concentration in a solution is direct and proportional. As the concentration of tryptophan in the solution increases, the absorbance of light by the solution also increases. This relationship is described by the Beer-Lambert Law, which states that absorbance is directly proportional to concentration.
What is the relationship between the wavelength of light and absorbance in a given substance?
The relationship between the wavelength of light and absorbance in a substance is that different substances absorb light at specific wavelengths. This absorption is measured as absorbance, which increases as the substance absorbs more light at its specific wavelength.
