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The multiple cloning sites (MCS) in pUC18 and pUC19 is the difference - the MCS in pUC19 is reverse orientated to those of the pUC18.
pUC18: LacZ HindIII PaeI ..... SacI EcoRI
pUC19: Lacz EcoRI SacI ..... PaeI HindIII
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∙ 15y ago
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