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The multiple cloning sites (MCS) in pUC18 and pUC19 is the difference - the MCS in pUC19 is reverse orientated to those of the pUC18.

pUC18: LacZ HindIII PaeI ..... SacI EcoRI

pUC19: Lacz EcoRI SacI ..... PaeI HindIII

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Q: What is the difference between Puc18 and Puc19?
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Related questions

What is the abbreviation of puc18?

puc18- plasmid of university of california 18


Is PUC18 a common YAC true or false?

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Puc18 is a vector or a plasmid?

PUC18 is a plasmid, specifically a commonly used cloning vector in molecular biology research. It is small in size and contains a multiple cloning site for inserting DNA fragments, as well as antibiotic resistance genes for selection in bacteria.


What enzyme is produced by cells transformed with plasmid lux that is not produced by cells transformed with pUC18?

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Why do the cells transformed with pUC18 and plasmid lux grow in the presence of ampicillin?

The pUC18 plasmid contains the ampicillin resistance gene (ampR) which confers resistance to ampicillin. The Lux operon on the plasmid allows for bioluminescence production and acts as a reporter gene. Therefore, transformed cells that harbor both plasmids can grow in the presence of ampicillin due to pUC18 and express bioluminescence due to the Lux operon.


What are the two different restriction enzymes used to cut the pUC19 plasmid and the lux gene DNA?

Two different restriction enzymes commonly used to cut the pUC19 plasmid are EcoRI and PstI. For cutting the lux gene DNA, the restriction enzymes commonly used are NcoI and HindIII.


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