Singular / plural.
Mark, please serve yourself before the potatoes get cold.
Janice, Georgia, Alice, please serve yourselves before the potatoes get cold.
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Themselves (plural)
Themself (singular) usually refers to an individual when gender is unknown.
'Themself' is a gender-neutral pronoun used for individuals who identify outside the gender binary, while 'themselves' is the reflexive pronoun used for plural subjects or to refer to one's own self.