Brittle
what colour does the clear alcohol become once the leaf is boiled in it
It does nothing apart from semi fermint the leaf
It really depends on the type of leaf and the color of the leaf.
The leaf was placed in alcohol wafter being placed in the boiling water to extract the green pigment from the leaf.
it turns green because the chlorophyll is evaporated out
by immersing it in a hot water bath
Ethanol dissolves chlorophyll hence further phtosynthetic activity is stopped in the abscence of light and the leaf becomes transparent (colorless). the colorless leaf takes better stain with iodene while testing for the presence of starch.
to denature the enzymes going to kill the leaf
if you cover a boiled leaf in iodine you can see how much starch is in the leaf
add the leaf to boiling ethanol in a water bath for a few minutes (the boiling ethanol dissolves the chlorophyll and removes the green colour from the leaf - it turns white so it is easy to see the change in colour) wash with water to rehydrate and soften the leaf
Alcohol is a solvent: It dissolves stuff. The leaf is porous, full of holes. When the leaf is placed in the alcohol, the alcohol gets into the leafs, and dissolves the pigments in the leaf, probably chlorophyll, which is green. This will turn the alcohol green.
Air. Basically that's the answer.