This depends on the final volume you intend on making. Say you want to prepare 500 mL of 1X TAE. You will need 10 mL of 50X TAE to prepare 500 mL of 1X TAE.
Usually you need to dilute from a 50x stock. On that case dilute: For 500mL: 10mL 50x TAE + 490mL H2O For 1L: 20mL 50x TAE + 980mL H2O
preparation of 500 ml 1x TAE buffer,50ml of 10x buffer add to 450ml DI.water. to calculate just use MV=MV so 500mL * 1x= 10x * V then solve for V. add the amount of DI water you need to get the volume you calculated above.
to make 500ml of 1x TAE solution we have to take 5ml of 100x TAE solution. mix it in 495 ml of deionized water.
0.04 M Tris-acetate, 0.001 M EDTA
Molarity of 1X PBS is 10mM. To prepare 20mM PBS take 16g of NaCl, 0.4 g KCl, 2.88 g Na2HPO4 0.48 g KH2PO4 in 800 ml of distilled H2O. Adjust the pH to 7.4 with HCl. Add H2O to 1 liter.
The stocks are commonly labeled as X factors such as 10X, 5X, 100X etc. X-factor indicates that the solution is concentrated and must be diluted usually with water to 1X concentration for use. For eg: - A 100X concentrated solution should be diluted to 100 fold. to convert 1X to 10X take one ml of 1x buffer in a measuring cylinder and dilute it to make it 10 ml. its now 10x buffer.
1 ml of 5X TE in 4ml distilled water (or).......if u want 100 ml just multiply 1 and 4 with 20....you will get 20 ml 5X TE in 80 ml distilled water
I think you mean electrphoresis buffer ( anyway the principle is the same for all sloutions,, simply it is chemistry ) if so , you'll need to take about 25 ml from your 40X buffer and complete them till 1000 ml with distilled water. The calculation can be done as the following N * V = N' * V' so 40X * Z = 1X * 1000 Z is the amount of 40X buffer needed to be diluted to give us 1L of 1X buffer so Z = 1000/40=25 ml
500% = 500/100 = 5.0 Answer #2 I disagree with the first answer. 1x is 100% 5x is 500% 50x is 5000% and 500x is 50000%
10x to 1x is a 1:10 dilution Therefore, add 1 part buffer, 9 parts DI-water If 100uL is 10uL (1 part buffer) and 90uL (9 parts DI-water) Then, 200ul (100 x 2) is 20uL (1 part buffer) and 180uL (9 parts DI-water)
50x (or any #x) is an indication of the speed of a drive. On a CD-ROM drive that is only capable of reading CDs, it is specifically referring to how fast the drive spins and reads the drive. 1x was the speed of the first CD-ROM drives (500 RPM and 150 KiB/s), and is still the speed used by most stereos when playing an audio CD. A 50x CD-ROM drive reads the disc approximately 50x faster than than the first drives.Other speeds can be labeled on a drive such as CD writing capabilities (how fast it can write to a disc based on the 500 RPM/150 KiB/s rate), DVD reading capability (based on speed relative to the first DVD drives) and writing DVDs (based on 1350 kB/s for 1x).
1x gb Alexander 1x gb andal 1x gb bestari 1x gb darius 1x gb dimicari 1x gb equeste 1x gb gaiodiaz 1x gb gyzarus 1x gb heraklinos 1x gb hoplomos 1x gb laquari 1x gb murmilo 1x gb ocatavius 1x gb retiari 1x gb samnite 1x gb secutor 1x gb spartacus 1x gb torax 2x test tiger 3x colosseum cage of the gladiator beasts 1x gb battle gladius 1x gb battle halberd 1x gb battle manica 2x defensive tactics 1x disarm 1x double tag team 2x parry 1x indominatable gb 1x Trojan gb 1x gb war chariot 1x gladiators proving ground you can add any limited or semi limited you want....hope this helps ...