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Southern Blotting refers to the identification of detailed sequences of DNA in which the DNA fragments are separated by electrophoresis

Northern Blotting refers to the identification of detailed sequences of RNA in which the RNA fragments are separated by electrophoresis

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Southern blotting is used to detect specific DNA sequences, while northern blotting is used to detect specific RNA sequences. Southern blotting involves DNA samples being digested with restriction enzymes, separated by gel electrophoresis, and then transferred to a membrane for probing. Northern blotting involves RNA samples being separated by gel electrophoresis, transferred to a membrane, and then probed with a complementary RNA or DNA sequence.

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Q: Difference between southern and northern blotting?
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SDS-PAGE is a technique used to separate proteins based on their size, while western blotting is a technique used to detect specific proteins in a sample using antibodies. In SDS-PAGE, proteins are separated by gel electrophoresis, while in western blotting, proteins are transferred from a gel to a membrane for detection using antibodies.


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