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Proteases: Any of various enzymes, including the endopeptidases and exopeptidases, that catalyze the hydrolytic breakdown of proteins into peptides or amino acids.
Poteinases: A protease that begins the hydrolytic breakdown of proteins, usually by splitting them into polypeptide chains. It is also a synonym of endopeptidases (e.g. pepsin, trypsin, or papain).
Note:
Protease - endo- & exo- peptidases; Catalyze hydrolytic breakdown
Proteinase - endo-peptidases; Begins hydrolytic breakdown by splitting
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What is the recommended proteinase K buffer recipe for optimal enzymatic activity in a biological sample?
The recommended proteinase K buffer recipe for optimal enzymatic activity in a biological sample typically includes Tris-HCl, calcium chloride, and sodium chloride. This buffer helps maintain the stability and activity of proteinase K, an enzyme that breaks down proteins in the sample.
What happens if you add too much proteinase k to a lysis buffer when performing DNA extraction?
Adding too much proteinase K can lead to excessive digestion of proteins in the sample, potentially reducing the effectiveness of subsequent DNA extraction steps. It can also result in degradation of the DNA itself, as proteinase K is an enzyme that can also digest DNA in high concentrations. It is important to carefully optimize the amount of proteinase K to prevent over-digestion of proteins and DNA.
What is the most effective method for inhibiting protease activity in a protease cocktail inhibitor?
The most effective method for inhibiting protease activity in a protease cocktail inhibitor is by using specific protease inhibitors that target and block the active sites of the proteases in the cocktail. These inhibitors can be designed to bind to the proteases and prevent them from functioning, thereby inhibiting their activity.
Why incubate the sample of DNA at 60 Celsius after adding the proteinase?
Incubating the DNA sample at 60 degrees Celsius after adding proteinase helps to degrade any contaminating proteins in the sample. The elevated temperature enhances the activity of the proteinase, leading to efficient digestion of proteins that could interfere with downstream applications such as PCR or sequencing. This step ensures that the DNA extracted is of high quality and free from protein impurities.
Why would a person who is unable to produce the enzyme protease benefit from eating pineapple or pawpaw?
Pineapple and pawpaw contain enzymes called bromelain and papain, respectively, which can help digest proteins in the absence of protease. By consuming these fruits, individuals lacking protease may find improved digestion of protein-rich foods.
