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3'-5' is a characteristic feature of DNA-polymerase I. This activity is meant to repair any misparing mistakes that the enzyme may commit during the synthesis, in which the enzyme would reverse its direction by ONE NUCLEOTIDE and excised the mistakenly added nucleotide, the enzyme acts at the phosphodiester bond at the 5 prime.

Whereas the 5'-3' exonuclease activity is an also repair strategy exercised by the DNA polymerase I. However, in this case the polymerase would move in the forwards direction and excise the miss-matched nucleotides at any position regardless with one nucleotide far or so many. This mechanism of repair is well documented in case UV-mutation.

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12y ago
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4mo ago

3' to 5' exonuclease activity refers to the ability of an enzyme to degrade DNA from the 3' end towards the 5' end, while 5' to 3' exonuclease activity degrades DNA in the opposite direction, from the 5' end towards the 3' end. These activities are important in processes like DNA repair, proofreading during DNA replication, and removal of RNA primers during DNA synthesis.

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Q: Difference Between 3' to 5' and 5' to 3' Exonuclease Activity?
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