Menses blood could possibly interfere with a home pregnancy kit. You can get a false positive or false negative with any testing kit. A women is better off seeing her general practitioner doctor or obstetrics/gynecological doctor for testing, pre-natal education and treatment.
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No, menstrual blood should not interfere with urine pregnancy test results. The hormone detected in a pregnancy test (hCG) is not present in menstrual blood, so the presence of menstrual blood should not impact the accuracy of the test.
You can take a home pregnancy test a few weeks after that night to check for pregnancy hormones in your urine, or visit a doctor for a blood test to confirm pregnancy. Symptoms like missed periods, breast tenderness, nausea, and fatigue can also indicate pregnancy, but the only way to be sure is through testing.
Pregnancy does not affect the accuracy of a pregnancy test. Pregnancy tests detect the presence of the hormone human chorionic gonadotropin (hCG) in the urine or blood, which is only present during pregnancy regardless of the woman's physiological state.
Fertilization is when the pregnancy hormone hCG has begun production and this hormone is what is searched for by pregnancy tests (urine and blood). However, this amount can be very little and may be difficult for a urine test to pick up. You can get the best accuracy a few days after your missed period, but they (urine tests) can detect up to 5 days before the first day of your last menstrual period. There is hCG during implantation, so it does have the potential to be detected and accurate, particularly for blood tests which are much more sensitive than urine tests.
Spotting refers to light bleeding that may occur in between menstrual periods. It is usually lighter and shorter in duration compared to a regular period. Spotting can have various causes, including hormonal fluctuations, pregnancy, birth control, infections, or certain medical conditions.
There are two kinds of blood tests for pregnancy tests -- quantitative and qualitative. Assuming you've waited long enough (much less than 49 days), and the test is done properly by the lab, the quantitative test is very accurate. The qualitative blood test is about the same accuracy as urine tests. Even the home urine tests are 97% accurate. So if you took the quantitative blood test you can be very sure it's right. Even with the other tests 97% is pretty accurate. I suspect the error rate in blood labs is greater than that.