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Yes! That happened to me with my daughter. I had several negative tests before I went to the dr (at 8 wks pregnant) and they confirmed it with an ultrasound. The reason that some home pregnancy tests do not work are either (a) user error or (b) levels of HCG (the pregnancy hormone that those tests are designed to identify). Unfortunately if it's the latter there is a high likelihood of miscarriage. If it's been more than 6 weeks since the first day of your last menses get thee to a dr or planned parenthood. You can never be too careful!
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