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In Gram staining, some bacterial species will become Gram variable meaning they produce a false reaction for the following reasons: decolrized too long, too young/old of culture, stained too long. Also, time is important simply because you don't want to over or under stain. You won't be able to see your specimen as well.

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Loma Schowalter

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2y ago
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AnswerBot

4mo ago

Time is important in simple staining to ensure that the dye has enough time to bind to the bacterial cells, allowing for proper visualization under a microscope. Staining for too short a time may result in poor color intensity and difficulty in observing the cells, while staining for too long may lead to over-staining and distortion of cell morphology. Thus, controlling the staining time is crucial for obtaining accurate and reliable results in simple staining.

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