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Why is an isotonic buffered extraction medium used initially to isolate the chloroplasts?
isotonic: to make sure the cells dont burst due to net flow of water into them that a hypertonic buffer can cause, or shrivel up, which a hypotonic buffer can cause.
buffered: to make sure the chloroplasts are kept at their physiological pH, which ensures that the key enzymes, proteins and other components of the cholorplasts retain their functionality.
What else can I help you with?
what can you use in place of barbitone buffer?
In place of barbitone buffer, you can use phosphate-buffered saline (PBS) or Tris-buffered saline (TBS) as alternatives. These buffers help maintain a stable pH and provide an isotonic environment for biological samples. However, the choice depends on the specific application, as different buffers can influence the behavior of proteins or nucleic acids during experiments. Always consider the compatibility with your particular assay or protocol.
Why use isotonic solution in DNA?
Isotonic solutions are used in DNA experiments to maintain the osmotic balance of cells and prevent them from bursting or shrinking due to osmotic pressure changes. This stability is crucial during processes like DNA extraction, where the integrity of the cell membrane must be preserved to effectively isolate DNA without damaging it. Additionally, isotonic conditions help ensure that cellular components remain intact for accurate analysis and manipulation of DNA.
What is an Isotonic Contractions?
Isotonic contractions. This happens when the muscle shortens as it contracts
Why is a sports drink used to extract the cheek cells?
its isotonic.
Is D5W Isotonic or Hypertonic?
Normal Saline Solution in 5% Dextrose or D5NSS is a hypertonic solution. It can be used for the temporary treatment of shock if plasma expanders are not available. However, do not administer this IV for clients with cardiac or renal conditions.
Is albumin isotonic?
isotonic
what can you use in place of barbitone buffer?
In place of barbitone buffer, you can use phosphate-buffered saline (PBS) or Tris-buffered saline (TBS) as alternatives. These buffers help maintain a stable pH and provide an isotonic environment for biological samples. However, the choice depends on the specific application, as different buffers can influence the behavior of proteins or nucleic acids during experiments. Always consider the compatibility with your particular assay or protocol.
Why use sodium chloride in Liver extraction?
Sodium chloride is used in liver extraction primarily to maintain osmotic balance and stabilize cell membranes during the process. It helps to prevent cell lysis by providing an isotonic environment, which is crucial for preserving the integrity of cellular structures. Additionally, sodium chloride can aid in the extraction of proteins and other biomolecules by solubilizing them, facilitating their subsequent analysis.
IS 5 dextrose in 0.9 saline isotonic?
.91 saline is normal saline which is isotonic. 5% dextrose is also isotonic. so the combination is still isotonic
Solutions that have the same osmotic pressure are called .?
Solutions having the same osmotic pressures are called isotonic solutions
Why use isotonic solution in DNA?
Isotonic solutions are used in DNA experiments to maintain the osmotic balance of cells and prevent them from bursting or shrinking due to osmotic pressure changes. This stability is crucial during processes like DNA extraction, where the integrity of the cell membrane must be preserved to effectively isolate DNA without damaging it. Additionally, isotonic conditions help ensure that cellular components remain intact for accurate analysis and manipulation of DNA.
How do isotonic solutions and equilibrium differ?
isotonic solution, equilibrium
To what body fluid is saline isotonic?
0.9% saline is isotonic to blood.
Use isotonic in a sentence?
I learn about isotonic in Biology. POSERS :D
What is an Isotonic Contractions?
Isotonic contractions. This happens when the muscle shortens as it contracts
Calculate the isotonic coefficient for NaCl if a 07M of NaCl solution equaled the hemolysis of14M glucose solution?
i = isotonic molar [glucose] / isotonic molar [NaCl] i = 14 M / 7 M = 2 i = isotonic molar [glucose] / isotonic molar [NaCl] i = 14 M / 7 M = 2 i = isotonic molar [glucose] / isotonic molar [NaCl] i = 14 M / 7 M = 2 i = isotonic molar [glucose] / isotonic molar [NaCl] i = 14 M / 7 M = 2
What does extraction buffer do?
The most likely function of the extraction buffer would be to maintain an isotonic environment that favors the stability of the protein. An isotonic solution mimics the ionic environment if the cell and therefore would keep the protein in a stable form during the process of extraction. Proteins undergo changes in different ionic environments (different pH's) and it is essential to keep them in a stable form.
