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To make sure the double-strand DNA template is separated into single strands.

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The initial denaturation step in PCR is done before the denaturation step to ensure that the DNA template is unwound and ready for amplification. This step helps to break down the secondary DNA structures and allows the primers to bind efficiently during the denaturation step, which is essential for the success of the PCR reaction.

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11mo ago
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Q: Why initial denaturation step is done before denaturation step in PCR?
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