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BlakeGlycerol is added to cetrimide agar as a carbon source to support the growth of Pseudomonas aeruginosa, a bacterium that uses glycerol as a substrate. Additionally, glycerol helps maintain the pH of the medium, ensuring optimal conditions for bacterial growth while inhibiting the growth of other organisms.
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Is cetrimide cream lipid soluble?
Yes, cetrimide cream is lipid soluble. Cetrimide, the active ingredient in the cream, is a quaternary ammonium compound that has surfactant properties and can dissolve in lipids. This property allows cetrimide cream to effectively penetrate the lipid-rich outer layer of the skin for various skin conditions.
Isolation media for actinomycetes?
Isolation media for actinomycetes typically contain nutrients like starch, casein, and soybean meal as carbon and nitrogen sources. Common isolation media include starch-casein agar, glycerol-asparagine agar, and oatmeal agar. Selective antibiotics like cycloheximide and nystatin may be added to inhibit the growth of other bacteria.
Why is agar added to nutrient media?
Agar is added to nutrient media as a solidifying agent. It provides a solid surface for bacterial or fungal growth to occur and can be easily sterilized. Agar also allows for the isolation and enumeration of colonies for microbiological analysis.
Is broth has agar?
Agar is a type of gelatinous substance obtained from seaweed, used as a culture medium in laboratories and as a thickening agent in food. Broth typically does not contain agar unless specifically added to it as a thickener.
Why is glycerol and blue dye added to the gel loading buffer during gel electrophoresis?
Glycerol is added to make the DNA sample denser so that it sinks into the gel and loads properly. Blue dye is added to visualize the sample loading and migration progress during electrophoresis.
