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Why does the crystal violent stain wash out of gram bacteria when they are decolorized in ethanol?
Wiki User
∙ 12y ago
gram negative have a very thin cell wall with an extra membrane layer (lipopolysac charide) out side.
Wiki User
∙ 12y ago
The crystal violet stain in Gram-positive bacteria forms a complex with the peptidoglycan layer in the cell wall, making it less permeable to decolorizer like ethanol. In Gram-negative bacteria, the outer membrane is disrupted by ethanol, allowing the crystal violet to be washed out during the decolorization step.
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