1) for uniform development
2) proper elution of sample
The chromatography chamber must be closed tightly to prevent the escape of solvents and vapors, which can affect the separation of compounds. It also helps to maintain consistent pressure and temperature inside the chamber, which is crucial for accurate and reproducible results.
The filter paper helps to evenly distribute the solvent vapor within the developing chamber, allowing for uniform separation of the components in the sample. It also acts as a medium for the sample to travel along with the solvent, facilitating the separation process in chromatography.
there are different types like gas chrom. and thinlayer chrom Answer: There are two types of chromatography:liquid chromatography gas chromatography
We use several things such as stationary phase which is a paper, mobile phase according to your solute and the mixture to be analysed. All these are placed in a closed chamber.
If the developing chamber is left uncovered during paper chromatography, the solvent will evaporate faster, leading to a higher concentration of the mobile phase at the starting line. This can result in shorter migration distances for the cations, leading to lower Rf values compared to when the chamber is covered and the conditions are more controlled.
The chromatography chamber must be closed tightly to prevent the escape of solvents and vapors, which can affect the separation of compounds. It also helps to maintain consistent pressure and temperature inside the chamber, which is crucial for accurate and reproducible results.
you need it in your body to live, so it is good for you.
it is cool
Covering the developing chamber tightly is important because it creates a saturated atmosphere, which allows for consistent and even solvent movement up the chromatography plate. This helps in achieving accurate separation of the compounds in the sample. Any gaps in the cover can lead to uneven solvent movement and affect the separation results.
The filter paper helps to evenly distribute the solvent vapor within the developing chamber, allowing for uniform separation of the components in the sample. It also acts as a medium for the sample to travel along with the solvent, facilitating the separation process in chromatography.
Synonyms for "saturate" : permeate, pervade, imbue, or soak.
there are different types like gas chrom. and thinlayer chrom Answer: There are two types of chromatography:liquid chromatography gas chromatography
1. thin -layer chromatography 2. gas chromatography 3. liquid chromatography
We use several things such as stationary phase which is a paper, mobile phase according to your solute and the mixture to be analysed. All these are placed in a closed chamber.
No, a heated solution will saturate faster.
chromatography or nitrogen containing compounds.....
If the developing chamber is left uncovered during paper chromatography, the solvent will evaporate faster, leading to a higher concentration of the mobile phase at the starting line. This can result in shorter migration distances for the cations, leading to lower Rf values compared to when the chamber is covered and the conditions are more controlled.