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You will do this regardless since Indole is part of the SIM media....this media will turn black if H2S is present and you read it for motility just like standard motility media. It must incubate for 24-28 hrs before doing the indole portion. Hope this helps.
Determining motility involves observing the movement of bacteria, which can be hindered by adding Kovac's reagent as this reagent can affect bacterial motility. Also, hydrogen sulfide production (H2S) is an important characteristic that can help differentiate bacterial species before adding Kovac's reagent, which is used to detect indole production. Waiting to add Kovac's reagent until after these tests ensures more accurate results.
because kovac's reagent is used for indole production test and for both mean indole and H2S test SIM Medium is used and it is possible to do both test together for one particular organism.
it is also possible that org. show both positive or both negetive.
if SIM Medium converk black mean H2S production and after kovac reagent addition if a red ring is formed mean indole production.
there is no requirement of any reagent addition for H2S TEST.
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Why anthrone reagent needs to be kept cold before using?
Anthrone reagent is prepared in concentrated sulfuric acid. Adding reagents to acid causes immediate dissociation of the reagent and releases heat. If your solution weren't cold, it could generate enough heat to blow out of the flask or bottle.
Why are solutions heated before evaporation?
well i THINK heating the solution will speed up the reaction in the solution before adding the precipitate since the final observation required is the precipitation. you dont add the reagent before heating since it may give a false observation after heating, it might not precipitate normally but since you've otherwise it might. BY : RAY LEE SEACREST
How is ninhydrine reagent prepared?
Ninhydrin reagent is prepared by dissolving ninhydrin powder in a solvent such as ethanol or acetone. The solution is typically heated gently to aid dissolution. It is important to prepare fresh ninhydrin reagent before use to ensure its effectiveness in detecting amino acids.
How is anthrone reagent stored?
Anthrone reagent should be stored in a tightly sealed container away from direct sunlight and heat sources, at room temperature. It should also be kept away from incompatible substances and moisture, and monitored for any signs of degradation or contamination before use.
Why the NaOH solution is heated before adding BaCl2 in the two solutions method for determination of purity of NaOH sample?
Why the NaOH is heated before adding in BaCl2 for determination of purity of NaOH sample?
Why anthrone reagent needs to be kept cold before using?
Anthrone reagent is prepared in concentrated sulfuric acid. Adding reagents to acid causes immediate dissociation of the reagent and releases heat. If your solution weren't cold, it could generate enough heat to blow out of the flask or bottle.
What is the limiting reagent when 150.0g of nitrogen react with 32.1 g of hydrogen?
To determine the limiting reagent, calculate the moles of each reactant: 150.0g nitrogen is 5.36 moles and 32.1g hydrogen is 31.8 moles. Using the balanced chemical equation, you can see that nitrogen is the limiting reagent because it will be completely consumed before all the hydrogen is reacted.
How do you prepare Schultz Reagent?
To prepare Schultz reagent, mix one part of concentrated sulfuric acid with nine parts distilled water. Allow the mixture to cool before use. This reagent is commonly used for the detection of proteins in biological samples.
How can you prepare borsches reagent in the lab?
To prepare Borsches reagent in the lab, mix equal volumes of concentrated sulfuric acid and glacial acetic acid in a fume hood. Then, slowly add 9 parts of water to the mixture while stirring constantly. Allow the reagent to cool before use.
HOW LONG ago is 480 BC from 2010?
480 BC was 2490 years before 2010. You can determine this by adding the current year to the BC year, 2010+480= 2490.
Do you have to estimate before adding?
No.
What did you learn from adding and subtracting fractions?
I learned to always change the denominators before adding or subtracting the numerators. You must always have a common denominator before adding or subtracting.
Why can't you prepare and store tollens' reagent in advance for tests?
Tollens' reagent is made by mixing two solutions - silver nitrate and ammonia - right before use because the reaction is sensitive to light, air, and temperature, which can degrade the reagent quickly. Preparing it in advance may lead to a loss of effectiveness, affecting the results of tests. Therefore, it is best to prepare Tollens' reagent fresh when needed for accurate testing.
Write the word in which the consonant was doubled before adding -ing?
Write the word in which the consonant was doubled before adding -ing.
Why are solutions heated before evaporation?
well i THINK heating the solution will speed up the reaction in the solution before adding the precipitate since the final observation required is the precipitation. you dont add the reagent before heating since it may give a false observation after heating, it might not precipitate normally but since you've otherwise it might. BY : RAY LEE SEACREST
Why is it important to simplify radical expressions before subtracting or adding and how is adding a radical expression similar to adding a polynomial expression and provide an example?
Why is it important to simplify radical expressions before adding or subtracting? How is adding radical expressions similar to adding polynomial expressions? How is it different? Provide a radical expression for your classmates to simplify..
What is the procedure to prepare anthrone reagent?
To prepare anthrone reagent, dissolve 200 mg of anthrone in 100 ml of concentrated sulfuric acid. Mix the solution thoroughly and allow it to cool before use. Anthrone reagent is used for the detection of carbohydrates, specifically sugars, in various biological samples.
