When to use 1x or 10x pbs?

Answer 1

Only use 1X PBS. 10X PBS is made up because it is easier to store and can be diluted when needed. Labs that use PBS often use it in large quantities so making up 10 or 20 liters at a time is usual and making it up as a concentrate is easier to do.

Answer 2

Use 1x PBS (phosphate-buffered saline) for routine washing and dilution steps in experiments. Use 10x PBS for preparing a concentrated stock solution that can be easily diluted to make 1x PBS when needed.