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Only use 1X PBS. 10X PBS is made up because it is easier to store and can be diluted when needed. Labs that use PBS often use it in large quantities so making up 10 or 20 liters at a time is usual and making it up as a concentrate is easier to do.

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15y ago
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6mo ago

Use 1x PBS (phosphate-buffered saline) for routine washing and dilution steps in experiments. Use 10x PBS for preparing a concentrated stock solution that can be easily diluted to make 1x PBS when needed.

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Q: When to use 1x or 10x pbs?
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Related questions

What does 1x PBS mean?

1X PBS might mean 1 in 10 solution 1/10


10x PBS buffer?

10x PBS buffer is a concentrated solution commonly used in biological and biochemical research. It typically contains a mixture of phosphate ions, sodium chloride, and potassium chloride at higher concentrations compared to the standard 1x PBS buffer. When diluted with water to 1x concentration, it can be used for a variety of applications such as cell culture, immunostaining, and protein analysis.


What is 10x - 1x?

10x - 1x = 9


How do you convert 1x buffer to 10x buffer?

To convert 1x buffer to 10x buffer, you would dilute the 1x buffer solution by adding 9 parts of a suitable solvent (such as water) to 1 part of the original buffer solution. This dilution will result in a 10x concentration of the buffer.


What is 1x PBS?

It is just Phosphate buffered saline.


What is the answer to 9x plus x?

9x + 1x = 10x.


What is the molarity of 1x PBS buffer and how to prepare 20 mM PBS buffer?

1x PBS buffer typically has a molarity of around 0.01 M. To prepare a 20 mM PBS buffer, you would need to dilute the 1x PBS stock solution with water. For example, to make 1 liter of 20 mM PBS buffer, you would need to mix 2 ml of 1 M PBS stock solution with 98 ml of water.


.1x equals 2 what is x?

incomplete formula.1x of 20 equals 2 = .10x is 0.1x?=2 ?=20


What is X if X plus .1X equals 110?

x+0.1x=110 10x+1x=1100 11x=1100 x=100


Help you with algebra -10x equals 80?

10x=80 divide each side by 10 to get x by itself 10/10x=1x 80/10=8 x=8


What is -11x-1 equals -10x-8?

-11x-1 = -10x-8 -11x+10 = -8+1 -1x = -7 x = 7


How do you dilute trypsin10x?

To dilute trypsin 10x, you can combine 1 part of the 10x concentrated trypsin solution with 9 parts of a suitable diluent, such as PBS or cell culture media. For example, add 1 mL of the 10x trypsin to 9 mL of the diluent to obtain a 1x trypsin solution. Mix thoroughly before use.