0
Synthesis of new DNA
Wiki User
∙ 13y ago
The reaction used to radioactively label DNA is typically performed using a DNA polymerase enzyme along with radioactive nucleotides, such as [α-32P]dNTPs. This allows for the incorporation of the radioactive label into the DNA strand during the polymerase reaction.
Add your answer:
Earn +20 pts
What enzyme us used to make radioactively labeled DNA?
DNA polymerase
What are radioactively tagged bacteriophages to discover that DNA not protein is injected into host cells?
Radioactively tagged bacteriophages are used to confirm that DNA, not protein, is injected into host cells during infection. The radioactively labeled DNA can be detected inside the host cells after infection, providing evidence that DNA is the genetic material transferred by the bacteriophages. This experiment was crucial in establishing DNA as the genetic material in organisms.
Can DNA polymerase be used to determine the general location of radioactively labeled DNA?
Geiger Counter
Can be used to compare the amount of radioactively labeled DNA in two or more samples?
Scintillation counter
The final step in the hybridization of radioactively DNA and normal DNA is to?
Make a solution with the two types of DNA
What can be used to radioactively label DNA?
Synthesis of new DNA
What is a following reactions that is used to radioactively label DNA?
Synthesis of new DNA.
What of the following reactions is used to radioactively label DNA?
The reaction that is commonly used to radioactively label DNA is the nick translation method, where a DNA molecule is treated with a DNA polymerase, dNTPs (including radioactive ones), and a DNAase to create radioactive labeled DNA fragments.
What enzyme us used to make radioactively labeled DNA?
DNA polymerase
Can DNA polymerase be used to determine the general location of radioactively labeled DNA?
Geiger Counter
Can be used to compare the amount of radioactively labeled DNA in two or more samples?
Scintillation counter
What are radioactively tagged bacteriophages to discover that DNA not protein is injected into host cells?
Radioactively tagged bacteriophages are used to confirm that DNA, not protein, is injected into host cells during infection. The radioactively labeled DNA can be detected inside the host cells after infection, providing evidence that DNA is the genetic material transferred by the bacteriophages. This experiment was crucial in establishing DNA as the genetic material in organisms.
The final step in the hybridization of radioactively DNA and normal DNA is to?
Make a solution with the two types of DNA
What reaction is used to radioactivley label DNA?
The reaction used to radioactively label DNA is the random primer labeling reaction. This involves using short oligonucleotide primers that hybridize randomly to the DNA template, which are then extended using a DNA polymerase in the presence of radioactive nucleotides.
Can be use to determine the precise amount of radioactively labeled DNA in a chemical solution?
scintillation counter. APEX
What kind of molecule must be labeled to make radioactively labeled DNA?
Radioactive nucleotide
Used to label DNA and protein in experiments?
Fluorescent dyes are commonly used to label DNA and protein in experiments. These dyes emit light when exposed to specific wavelengths of light, allowing for visualization and analysis of the labeled molecules. Additionally, radioactive isotopes can also be used for labeling, but they may pose safety and disposal challenges.
