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The reaction used to radioactively label DNA is typically performed using a DNA polymerase enzyme along with radioactive nucleotides, such as [α-32P]dNTPs. This allows for the incorporation of the radioactive label into the DNA strand during the polymerase reaction.
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What enzyme us used to make radioactively labeled DNA?
DNA polymerase
What are radioactively tagged bacteriophages to discover that DNA not protein is injected into host cells?
Radioactively tagged bacteriophages are used to confirm that DNA, not protein, is injected into host cells during infection. The radioactively labeled DNA can be detected inside the host cells after infection, providing evidence that DNA is the genetic material transferred by the bacteriophages. This experiment was crucial in establishing DNA as the genetic material in organisms.
Can DNA polymerase be used to determine the general location of radioactively labeled DNA?
Geiger Counter
Can be used to compare the amount of radioactively labeled DNA in two or more samples?
Scintillation counter
The final step in the hybridization of radioactively DNA and normal DNA is to?
Make a solution with the two types of DNA
What can be used to radioactively label DNA?
Synthesis of new DNA
What of the following reactions is used to radioactively label DNA?
The reaction that is commonly used to radioactively label DNA is the nick translation method, where a DNA molecule is treated with a DNA polymerase, dNTPs (including radioactive ones), and a DNAase to create radioactive labeled DNA fragments.
What is a following reactions that is used to radioactively label DNA?
Synthesis of new DNA.
What reaction is used to radioactivley label DNA?
The reaction used to radioactively label DNA is the random primer labeling reaction. This involves using short oligonucleotide primers that hybridize randomly to the DNA template, which are then extended using a DNA polymerase in the presence of radioactive nucleotides.
What enzyme us used to make radioactively labeled DNA?
DNA polymerase
What are radioactively tagged bacteriophages to discover that DNA not protein is injected into host cells?
Radioactively tagged bacteriophages are used to confirm that DNA, not protein, is injected into host cells during infection. The radioactively labeled DNA can be detected inside the host cells after infection, providing evidence that DNA is the genetic material transferred by the bacteriophages. This experiment was crucial in establishing DNA as the genetic material in organisms.
Can DNA polymerase be used to determine the general location of radioactively labeled DNA?
Geiger Counter
Can be used to compare the amount of radioactively labeled DNA in two or more samples?
Scintillation counter
How do radioisotopes identify DNA?
Radioisotopes are often used to label DNA molecules for detection purposes. This is achieved by incorporating radioactively labeled nucleotides during DNA replication or using radiolabeled probes that bind specifically to the DNA sequence of interest. By detecting the emitted radiation from the radioisotope, researchers can locate and identify the DNA molecules.
The final step in the hybridization of radioactively DNA and normal DNA is to?
Make a solution with the two types of DNA
You briefly expose bacteria undergoing DNA replication to radioactively labeled nucleotides when you centrifuge the DNA isolated from the bacteria the DNA separates into two classes one class of la?
The DNA separated into two classes: labeled DNA and unlabeled DNA. The labeled DNA contains the radioactively labeled nucleotides that were incorporated during DNA replication, while the unlabeled DNA represents the original, non-radioactively labeled DNA from the bacteria. The centrifugation process separated the DNA based on density, with the heavier labeled DNA migrating to a higher position in the centrifuge tube compared to the unlabeled DNA.
Can be use to determine the precise amount of radioactively labeled DNA in a chemical solution?
scintillation counter. APEX
