They might have concluded that both protein and DNA transmitted hereditary material. The 32P was a radioisotope in DNA, and the 35S was a radioisotope in proteins. They were used to track the DNA and proteins to see if they were injected by a virus into a bacterium.
Hershey and Chase's experiment using bacteriophages proved that DNA, not protein, is the genetic material in cells. This discovery was a key milestone in understanding how genetic information is passed from one generation to the next. It laid the foundation for the field of molecular biology.
They found that all of the viral DNA and little of the protein had entered E. coli cells. Then they concluded that DNA is the hereditary molecule in viruses.
Hershey and Chase knew that the particular phage they worked with has two basic components: DNA on the inside, coated with protein on the outside. In their expt theylabelled phages with radioisotopes . For one batch of infecting phages, they used a radioactive isotope of sulfur to label only the phages' protein coats.In another batch of phages, they used a radioactive isotope of phosphorus to label only DNA. Next, they allowed each batch of phages to infect separate cultures of nonradioactive bacterial cells. They then whirled each culture in a blender to shake loose any parts of the phages that remained outside the bacterial cells. Result of expt. confirmed that only DNA of phage entered bacteria .
It didn't. The DNA ended up in the pellet-like bacteria that was at the bottom of the centrifuge. The protein capsids ended up in the supernatant because they weren't injected into the bacteria cells. The supernatant was separated from the bacteria cells because the bacteria was heavier and fell to the bottom.
My biology book says: Hershey and Chase's experiment with bacteriophages confirmed Avery's results, convincing many scientists that DNA was the genetic material found in genes- not just in viruses and bacteria, but in all living cells. Hoped this helps :)
Hershey and Chase's experiment using bacteriophages proved that DNA, not protein, is the genetic material in cells. This discovery was a key milestone in understanding how genetic information is passed from one generation to the next. It laid the foundation for the field of molecular biology.
They found that all of the viral DNA and little of the protein had entered E. coli cells. Then they concluded that DNA is the hereditary molecule in viruses.
They found that all of the viral DNA and little of the protein had entered E. coli cells. Then they concluded that DNA is the hereditary molecule in viruses.
They might have concluded that both protein and DNA transmitted hereditary material. The 32P was a radioisotope in DNA, and the 35S was a radioisotope in proteins. They were used to track the DNA and proteins to see if they were injected by a virus into a bacterium.
The scientists used a blender to break open the bacterial cells infected by phages, releasing the phage DNA. This demonstrated that the phage DNA carries instructions to produce new phage particles when introduced into a host bacterial cell.
They found that all of the viral DNA and little of the protein had entered E. coli cells. Then they concluded that DNA is the hereditary molecule in viruses.
Hershey and Chase radioactively labelled T2 bacteriophage with P (DNA) and S (protein) to determine if protein or DNA carried the genetic material. They let the bacteriophage infect E.coli. The radioactive material found in the host E.coli contained the radioactive P (found in DNA) as the bacteriophage had multiplied inside the cell, but the radioactive S was not found inside the cell. Therefore DNA is the hereditary molecule resorce - some other guy off the internet
Hershey and Chase knew that the particular phage they worked with has two basic components: DNA on the inside, coated with protein on the outside. In their expt theylabelled phages with radioisotopes . For one batch of infecting phages, they used a radioactive isotope of sulfur to label only the phages' protein coats.In another batch of phages, they used a radioactive isotope of phosphorus to label only DNA. Next, they allowed each batch of phages to infect separate cultures of nonradioactive bacterial cells. They then whirled each culture in a blender to shake loose any parts of the phages that remained outside the bacterial cells. Result of expt. confirmed that only DNA of phage entered bacteria .
The genetic mateial is made of DNA and not of proteins
Hershey and Chase chose to use viruses in their experiments because viruses are simple and can easily infect and replicate within host cells, making them ideal for studying the process of genetic material transfer.
In their experiments, Hershey and Chase injected chemical tracers that resembled proteins and DNA into a type of rapidly reproducing virus called T2. They then tracked the course of the tracers as they made their way through reproduction and found that the tracers that resembled DNA were much more likely than the tracers that resembled proteins to be found in future generations of the cells. Hershey and Chase concluded that DNA, and not protein, was associated with genetic material.
They used the T2 phage in their Blender experiment.