They might have concluded that both protein and DNA transmitted hereditary material. The 32P was a radioisotope in DNA, and the 35S was a radioisotope in proteins. They were used to track the DNA and proteins to see if they were injected by a virus into a bacterium.
Hershey and Chase's experiment using bacteriophages proved that DNA, not protein, is the genetic material in cells. This discovery was a key milestone in understanding how genetic information is passed from one generation to the next. It laid the foundation for the field of molecular biology.
They found that all of the viral DNA and little of the protein had entered E. coli cells. Then they concluded that DNA is the hereditary molecule in viruses.
Hershey and Chase knew that the particular phage they worked with has two basic components: DNA on the inside, coated with protein on the outside. In their expt theylabelled phages with radioisotopes . For one batch of infecting phages, they used a radioactive isotope of sulfur to label only the phages' protein coats.In another batch of phages, they used a radioactive isotope of phosphorus to label only DNA. Next, they allowed each batch of phages to infect separate cultures of nonradioactive bacterial cells. They then whirled each culture in a blender to shake loose any parts of the phages that remained outside the bacterial cells. Result of expt. confirmed that only DNA of phage entered bacteria .
My biology book says: Hershey and Chase's experiment with bacteriophages confirmed Avery's results, convincing many scientists that DNA was the genetic material found in genes- not just in viruses and bacteria, but in all living cells. Hoped this helps :)
In the Hershey-Chase experiments, DNA ended up in the supernatant because it is a large molecule that remained inside the bacterial cells, while the protein coat of the phage (which was labeled with radioactive sulfur) was stripped off and left outside the cell. This separation allowed researchers to conclude that DNA is the genetic material responsible for viral replication.
Hershey and Chase's experiment using bacteriophages proved that DNA, not protein, is the genetic material in cells. This discovery was a key milestone in understanding how genetic information is passed from one generation to the next. It laid the foundation for the field of molecular biology.
They found that all of the viral DNA and little of the protein had entered E. coli cells. Then they concluded that DNA is the hereditary molecule in viruses.
They might have concluded that both protein and DNA transmitted hereditary material. The 32P was a radioisotope in DNA, and the 35S was a radioisotope in proteins. They were used to track the DNA and proteins to see if they were injected by a virus into a bacterium.
They found that all of the viral DNA and little of the protein had entered E. coli cells. Then they concluded that DNA is the hereditary molecule in viruses.
The scientists used a blender to break open the bacterial cells infected by phages, releasing the phage DNA. This demonstrated that the phage DNA carries instructions to produce new phage particles when introduced into a host bacterial cell.
They found that all of the viral DNA and little of the protein had entered E. coli cells. Then they concluded that DNA is the hereditary molecule in viruses.
Hershey and Chase conducted the famous Hershey-Chase experiment in 1952, which confirmed that DNA, not protein, is the genetic material in viruses. They used radioactive labeling to track the transmission of genetic information during viral infection of bacterial cells. This provided crucial evidence supporting the role of DNA as the carrier of genetic information.
Hershey and Chase knew that the particular phage they worked with has two basic components: DNA on the inside, coated with protein on the outside. In their expt theylabelled phages with radioisotopes . For one batch of infecting phages, they used a radioactive isotope of sulfur to label only the phages' protein coats.In another batch of phages, they used a radioactive isotope of phosphorus to label only DNA. Next, they allowed each batch of phages to infect separate cultures of nonradioactive bacterial cells. They then whirled each culture in a blender to shake loose any parts of the phages that remained outside the bacterial cells. Result of expt. confirmed that only DNA of phage entered bacteria .
The genetic mateial is made of DNA and not of proteins
Hershey and Chase chose to use viruses in their experiments because viruses are simple and can easily infect and replicate within host cells, making them ideal for studying the process of genetic material transfer.
Hershey and Chase's experiment using T2 bacteriophages demonstrated that DNA, not protein, is the genetic material responsible for the inheritance of traits in organisms. This experiment provided strong evidence supporting the role of DNA as the molecule that carries genetic information and can be replicated and passed on from generation to generation.
They used the T2 phage in their Blender experiment.