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What is the restriction site of the restriction enzyme Hae III?
The restriction site of the restriction enzyme Hae III is GGCC. It recognizes and cuts this site in DNA, producing 4-base pair overhangs (sticky ends).
What is the restriction site to restriction enzyme Hae III?
The restriction site for the restriction enzyme Hae III is GGCC. Hae III recognizes this specific sequence and cuts the DNA at this site.
What will cut a DNA sequence only if it matches the sequence precisely?
A restriction enzyme will cut a DNA sequence only if it matches the specific recognition sequence of that enzyme. These enzymes are highly specific and will cleave the DNA at a particular site when the target sequence is present in the DNA molecule.
What is Example of restriction enzyme?
A restriction enzyme (also known as restriction endonuclease) is protein which cuts DNA up at specific sequences (called restriction sites) in a genome. For example, the commonly used restriction endonuclease EcoRI recognizes every DNA sequence GAATTC and cuts at the point between the guanine and the adenine in that sequence, forming blunt ends (or straight, even ends). Interestingly and coincidentially, the restriction site for most restriction enzymes are genetic palindromes (the sequence reads exactly the same backwards on the complementary strand). In the case of EcoRI, the two complementary DNA strands for the restriction site are:5'-- GAATTC --3'3'-- CTTAAG --5'After this DNA sequence is cut, it might look something like this:5'-- G AATTC --3'3'-- C TTAAG --5'
Why did you cut both segments of DNA with the same restriction enzyme?
You use the same enzyme inn order to get the same restriction and binding sites.
What is the restriction site of the restriction enzyme Hae III?
The restriction site of the restriction enzyme Hae III is GGCC. It recognizes and cuts this site in DNA, producing 4-base pair overhangs (sticky ends).
What is the restriction site to restriction enzyme Hae III?
The restriction site for the restriction enzyme Hae III is GGCC. Hae III recognizes this specific sequence and cuts the DNA at this site.
Where in the DNA sequence does the restriction enzyme EcoR1 specifically cut?
The restriction enzyme EcoR1 specifically cuts the DNA sequence at the recognition site GAATTC.
What is a palindrome as it relates to a restriction enzyme site?
A palindrome in the context of a restriction enzyme site refers to a sequence of DNA that reads the same forward and backward. Many restriction enzymes recognize and cut DNA at palindromic sequences. This characteristic allows the enzyme to bind symmetrically to both strands of DNA.
What is the specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site?
The specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site, is 5'-GAATTC-3'.
What is the term that describes the sequence of DNA that a restriction enzyme finds and cuts?
Recognition sequence.
What is a DNA restriction site?
A DNA restriction site is a specific short nucleotide sequence recognized by a restriction enzyme, which cuts the DNA at that site. These sites are often palindromic, meaning the sequence reads the same forwards and backwards. Restriction sites are commonly used in molecular biology for DNA manipulation and analysis.
Why does raising the temperature to 42 degrees stop the enzyme restriction?
Raising the temperature to 42 degrees can denature the enzyme, changing its shape and disrupting its active site. This can prevent the enzyme from functioning properly, halting the restriction process.
What determines how DNA will be cut by a restriction enzyme?
Restriction enzymes recognize specific DNA sequences called restriction sites, typically palindromic sequences. When a restriction enzyme encounters its specific recognition site, it binds to the DNA and cuts it at a specific location within or near the recognition site. The specific sequence and structure of the recognition site determine how the DNA will be cut by the restriction enzyme.
An enzyme that cuts double-stranded DNA at specific nucleotide sequences?
This enzyme is called a restriction enzyme. They are commonly used in molecular biology to create DNA fragments with specific ends that can be ligated together.
What is the best explanation for why a restriction enzyme does not cut the DNA of the cell that produces it?
Restriction enzymes are made by cells to protect their own DNA from being cut. These cells produce a modification enzyme that adds a methyl group to specific sites on their own DNA sequence, which prevents the restriction enzyme from cutting. This process is known as "methylation protection."
Which restriction enzyme did you use to cut the DNA?
The restriction enzyme used to cut the DNA was EcoRI.
