0
Still curious? Ask our experts.
Chat with our AI personalities
Steve
Beau
Blake
Add your answer:
Earn +20 pts
What is the restriction site of the restriction enzyme Hae III?
The restriction site of the restriction enzyme Hae III is GGCC. It recognizes and cuts this site in DNA, producing 4-base pair overhangs (sticky ends).
What is the restriction site to restriction enzyme Hae III?
The restriction site for the restriction enzyme Hae III is GGCC. Hae III recognizes this specific sequence and cuts the DNA at this site.
What will cut a DNA sequence only if it matches the sequence precisely?
A restriction enzyme will cut a DNA sequence only if it matches the specific recognition sequence of that enzyme. These enzymes are highly specific and will cleave the DNA at a particular site when the target sequence is present in the DNA molecule.
What is Example of restriction enzyme?
A restriction enzyme (also known as restriction endonuclease) is protein which cuts DNA up at specific sequences (called restriction sites) in a genome. For example, the commonly used restriction endonuclease EcoRI recognizes every DNA sequence GAATTC and cuts at the point between the guanine and the adenine in that sequence, forming blunt ends (or straight, even ends). Interestingly and coincidentially, the restriction site for most restriction enzymes are genetic palindromes (the sequence reads exactly the same backwards on the complementary strand). In the case of EcoRI, the two complementary DNA strands for the restriction site are:5'-- GAATTC --3'3'-- CTTAAG --5'After this DNA sequence is cut, it might look something like this:5'-- G AATTC --3'3'-- C TTAAG --5'
Why did you cut both segments of DNA with the same restriction enzyme?
Cutting both segments with the same restriction enzyme ensures that they will have compatible ends that can be easily ligated together. This allows for the creation of a recombinant DNA molecule.
