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Egg Infected dose 50% Vs. Tissue Culture Infectious Dose 50%

Two ways of testing for virus. One with eggs and one with tissue.

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17y ago

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How do you administrate TCID50 and EID50?

When you mean administrate, does this mean procedure for TCID50 or calculation for use? In calculating for the TCID50 and EID50 of the virus in question, you can either use the Spearman-Karber formula or the Reed amd Muench formula. You will be able to determine the amount of virus per 0.1 ml or 1.0 ml depending on the amount used in the procedure. There are a lot of procedure available in the net for both tests. If the computation is 105 per 0.1 ml then there are 100,000 virus particles in that volume. If you need only 103 per ml then 1.0 ml the original suspension shall be diluted with equal volume of diluent (1.0 ml.)


Is tcid50 the same as viral titer?

Tes categorically speaking yes. The titer of the virus using the TCID50 procedure shall be expressed as the titre of the virus


How do you calculate for eid50?

The Eid50, or effective infectious dose for 50% of a population, is calculated using dose-response data, typically obtained from experiments involving a range of doses administered to a population. You plot the proportion of individuals infected or affected against the logarithm of the dose, and then fit a sigmoidal curve to the data. The Eid50 is the dose at which 50% of the population exhibits the response or infection. Statistical software or models, such as the probit or logit model, can be used to analyze the data and determine the Eid50 value.


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