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Egg Infected dose 50% Vs. Tissue Culture Infectious Dose 50%

Two ways of testing for virus. One with eggs and one with tissue.

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16y ago
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1mo ago

EID50 (50% endpoints infectious dose) is used to measure the amount of virus causing infection in the host, while TCID50 (50% tissue culture infectious dose) is used to measure virus infectivity in cell culture. They both represent the viral titer required to produce a specific response (infection) in a biological system, but EID50 is usually used for animal models, while TCID50 is used for experiments conducted in cell cultures.

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Q: What is the relation between EID50 and TCID50?
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How do you administrate TCID50 and EID50?

When you mean administrate, does this mean procedure for TCID50 or calculation for use? In calculating for the TCID50 and EID50 of the virus in question, you can either use the Spearman-Karber formula or the Reed amd Muench formula. You will be able to determine the amount of virus per 0.1 ml or 1.0 ml depending on the amount used in the procedure. There are a lot of procedure available in the net for both tests. If the computation is 105 per 0.1 ml then there are 100,000 virus particles in that volume. If you need only 103 per ml then 1.0 ml the original suspension shall be diluted with equal volume of diluent (1.0 ml.)


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