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The chromatography paper is spotted twice so as to increase the amount of sample used for the separation on the paper. This is to allow distinct banding of the sample into its constituents.

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Double-spotting chromatography paper helps ensure that the substance being analyzed is evenly distributed across the paper to enhance separation and analysis. It can also be used as a reference spot to track the movement of the solvent front during the chromatography process.

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9mo ago
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Q: What is the purpose of double spotting the chromatography paper?
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