What is the function of isopropanol in DNA extraction?

Answer 1

To concentrate or purify the DNA, which is insoluble in isopropanol. Once the solution containing your DNA is placed in isopropanol and centrifuged, the DNA will precipitate to a little pellet at the bottom of your tube. Everything else in your tube is soluble in isopropanol and will remain in liquid form. Pipet the liquid out and now you have just DNA.

Answer 2

Isopropanol is used in DNA extraction to precipitate DNA out of solution. When added to a DNA-containing solution, isopropanol causes DNA molecules to come out of solution and form a visible clump, making it easier to separate the DNA from other cellular components.

Answer 3

DNA is insoluble in isopropanol and therefore, it is ideal for DNA extraction because it allows the DNA to precipitate. The isopropanol is also ice cold because it helps keep the DNA in shape so that it can be readily observed.

Answer 4

this is a point I have got when i searched on internet. eventhough the isopropanol is less efficient than ethanol in precipitating rna, in presence of NH4 cations it is better than ethanol to keep free nucleotide in solution and so separating then from precipitated rna.

Answer 5

Isopropanol effectively precipitates nucleic acids,process is called salting out of DNA. but is much less effective with proteins. A quick precipitation can therefore purify DNA from protein contaminants.

Answer 6

DNA is very insoluble in alcohols. When isopropanol or ethanol are mixed with a DNA containing solution, the DNA molecule in the solution aggregates and precipitate out. By this way one can concentrate DNA to the desired volume.

And isopopanol is mainly used because, it evaporates easily(after precipitation) and precipitates DNA much better than ethanol.

Answer 7

precipitates the DNA out of solution so it can be spooled/hooked and redissolved in TE buffer.

Answer 8

for the precipitation of RNA present in aqueous medium.

Answer 9

Alcohol makes dna precipitate leaving the impurities( Proteins etc) in the soluble form.

Answer 10

The reason why isoproponal is used in DNA extraction is because it is stronger at precipitating the DNA. DNA is also insoluble in isoproponal and is cold to help the DNA retain its shape.