TBE (Tris Borate EDTA).. TBE usually use like TAE in electrophoresis.. The difference is the size of molecule DNA that you want to separate. You can use TBE for the small size than TAE.
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∙ 2010-10-20 15:31:32To achieve precipitation DNA.
In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
DTT is the reducing agent for thiolated DNA
In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
Sodium chloride help to separate DNA from other proteins.
To achieve precipitation DNA.
Cold water helps keep the DNA intact during extraction.
In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
chelating Mg2+
It solubalize lipids and protiens to remove them from DNA
Sodium chloride help the the precipitation of DNA.
DTT is the reducing agent for thiolated DNA
In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
removal of polyphenols
maintain ions in buffer....
It lyses the cell membrane and nucleus to make the extraction possible
ammonium acetae use to percipitate DNA from water.