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When you increase the flowrate of the carrier gas, the retention times decrease. Just like when you increase the temperature of the column. Both of these conditions are sometimes necessary for substances that would otherwise have very long retention times.
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∙ 16y ago
In general, a higher flow rate in HPLC can lead to shorter retention times due to decreased interaction time between the analyte and the stationary phase. However, excessively high flow rates can negatively impact peak resolution and efficiency. It is important to optimize the flow rate to achieve the best separation performance for a given set of conditions.
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∙ 14y agowhy HPLC dependent on flow rate & retention time
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Why ordinary liquid pump cannot be used in hplc system?
An ordinary liquid pump cannot be used in HPLC systems because HPLC requires pumps that can generate high pressure to push solvents through the column at a constant and precise flow rate. Ordinary pumps do not provide the level of pressure and flow rate control needed for HPLC separations, which can negatively impact the accuracy and reproducibility of chromatographic results. HPLC pumps are specifically designed to handle the high pressures and flow rates required for efficient separations.
How to fix concentration of RS by HPLC?
To fix retention time shifts (RS) in HPLC, you can try the following: Check for column stability and ensure it is properly equilibrated. Optimize mobile phase composition and flow rate to improve peak sharpness. Verify sample preparation and injection conditions to ensure consistency. Regularly maintain and calibrate the HPLC system to ensure accurate results.
Why pressure is important in HPLC?
Pressure is important in HPLC to maintain the flow rate of the mobile phase through the column, which is necessary for efficient separation of compounds. The pressure also helps to ensure that the analytes are pushed through the column at a constant rate, resulting in consistent and reliable chromatographic results. Adequate pressure is needed to overcome frictional forces within the system and to prevent band broadening.
What is flow rate in gc?
In gas chromatography, flow rate refers to the rate at which the carrier gas flows through the chromatographic system. It plays a crucial role in determining the efficiency of the separation process by affecting the retention time and resolution of analytes. Flow rate is typically measured in milliliters per minute (mL/min) or cubic centimeters per minute (cc/min).
Can relative retention time changed?
Yes, relative retention time can change based on various factors such as changes in column temperature, flow rate, and mobile phase composition. These changes can affect the interaction between the analyte and stationary phase, thereby altering the relative retention time.
Why ordinary liquid pump cannot be used in hplc system?
An ordinary liquid pump cannot be used in HPLC systems because HPLC requires pumps that can generate high pressure to push solvents through the column at a constant and precise flow rate. Ordinary pumps do not provide the level of pressure and flow rate control needed for HPLC separations, which can negatively impact the accuracy and reproducibility of chromatographic results. HPLC pumps are specifically designed to handle the high pressures and flow rates required for efficient separations.
What are the HPLC Calibration parameters and elaborate it?
1. Flow rate 2. Temp. of column 3. Detector function 4. Resolution
What does lake retention time refer to?
Lake retention time refers to measurements based on the volume of water in a lake and the average rate of outflow. It represents the amount of time it takes for a substance introduced into a lake to flow out of it again.
What is the effect that the flow radius change has on flow rate?
Increasing the flow radius generally leads to an increase in flow rate, as there is more cross-sectional area for fluid to flow through. Conversely, decreasing the flow radius usually results in a decrease in flow rate due to the reduction in available space for fluid passage.
What is flow rate in gc?
In gas chromatography, flow rate refers to the rate at which the carrier gas flows through the chromatographic system. It plays a crucial role in determining the efficiency of the separation process by affecting the retention time and resolution of analytes. Flow rate is typically measured in milliliters per minute (mL/min) or cubic centimeters per minute (cc/min).
How do you calculate employee retention rate?
retentiontime = volume of object/flow rate through objectso, in our example:retentiontime = 500 (gpm) / 2000 (gpm / hour flow rate) = 0.25 hours (which is 15 minutes).so a given sample of water will take 15 minutes to pass through the object
Can relative retention time changed?
Yes, relative retention time can change based on various factors such as changes in column temperature, flow rate, and mobile phase composition. These changes can affect the interaction between the analyte and stationary phase, thereby altering the relative retention time.
How do you calculate client retention rate?
What is your customer retention rate? Number of current customers __5086___. Total number of customers served in the past 12 months:__256___. Customer Retention Rate = #1 ÷ #2 = _____ (your percentage of retention). What is your goal for your customer retention rate this year? _____ *It is important to measure the change in this number with regularity. *When it is below 80%, then it is a serious area of concern.
What is the function of pump in HPLC?
The pump in HPLC is responsible for delivering the mobile phase at a constant and precise flow rate through the column. This ensures consistent and reproducible separation of compounds in the sample. The pump helps maintain the pressure necessary for optimal chromatographic performance.
What effect do you think increasing the flow tube length will have on the fluid flow rate?
Increasing the flow tube length will typically result in a decrease in the fluid flow rate. This is because the longer flow tube increases the resistance to flow, causing a reduction in the flow rate of the fluid passing through it.
What is the effect of pump installation in series and parallel connection?
if you connect the pumps in series, it will add head at the same flow rate. Inversely, in parallel connection, double the flow rate at same head.
How does changing the temperature of a fluid effect the flow rate of the fluid?
Increasing the temperature of a fluid typically decreases its viscosity, making it flow more easily. This reduced viscosity can lead to an increase in flow rate as the fluid encounters less resistance while flowing. Conversely, decreasing the temperature usually increases the fluid's viscosity, resulting in a decrease in flow rate due to increased resistance to flow.
